| 丝素/SiO2杂化膜固定化多酚氧化酶处理模拟含酚废水 |
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| 引用本文： | 王亚玲,习霞,杨宇民,邵健. 丝素/SiO2杂化膜固定化多酚氧化酶处理模拟含酚废水[J]. 环境污染与防治, 2009, 31(3) |
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| 作者姓名： | 王亚玲 习霞 杨宇民 邵健 |
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| 作者单位： | 南通大学化学化工学院,江苏南通,226001;江苏省神经再生重点实验室,江苏,南通,226001 |
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| 基金项目： | 国家自然科学基金，南通市应用研究计划项目 |
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| 摘 要： | 粗马铃薯多酚氧化酶固定于丝素/SiO2杂化膜上,从而得到丝素/SiO2杂化膜固定化多酚氧化酶.并用于处理模拟含酚废水.丝素/SiO2杂化膜在水中的溶失率小于丝素膜在水中的溶失率.所得丝素/SiO2杂化膜固定化多酚氧化酶的最适宜pH为7.4.相对于游离多酚氧化酶.该酶具有较好的热稳定性、贮存稳定性及重复使用性.研究表明,丝素/SiO2杂化膜固定化多酚氧化酶有实际处理含酚废水的可能.
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| 关 键 词： | 多酚氧化酶 丝素 固定化酶 苯酚 |
Immobilization of polyphenol oxidase on silk/SiO_2 membrane for treating phenolic wastewater |
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| Wang Yaling,Xi Xia,Yang Yumin,Shao Jian. Immobilization of polyphenol oxidase on silk/SiO_2 membrane for treating phenolic wastewater[J]. Environmental Pollution & Control, 2009, 31(3) |
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| Authors: | Wang Yaling Xi Xia Yang Yumin Shao Jian |
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| Affiliation: | 1.College of Chemistry and Chemical Engineering;Nantong University;Nantong Jiangsu 226001;2.Jiangsu Province Key Laboratory of Neuroregeneration;Nantong Jiangsu 226001 |
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| Abstract: | A hybrid membrane of silk fibroin/SiO2(SF/SiO2) was prepared to retain the partially purified potato polyphenol oxidase(PPO) for removing a phenolic compound from the test solution.The hybrid membrane was less water soluble than the SF membrane.The optimum pH of immobilized PPO was about 7.4.This immobilized PPO exhibited a higher thermal stability,and it was more stable and more effective for continuous use than the free PPO.The SF/SiO2 membrane with immobilized PPO was reusable after regeneration by water... |
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| Keywords: | polyphenol oxidase silk fibroin immobilized enzyme phenol |
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