谷氨酰胺转胺酶（MTG）的中试生产条件

考察了5L罐中淀粉预处理方式对微生物谷氨酰胺转胺酶（MTG）酶活的影响，探讨了MTG中试生产过程的逐级放大原则，并将5L小罐实验结果放大到30L小试和300L中试规模，研究结果表明：采用经液化处理的工业淀粉培养基进行发酵，MTG酶活、MTG生产强度及MTG产率分别比未用液化淀粉培养基提高了32%，32%和70%；以单位培养液体积中空气流量（qv/V）相同及消耗的功率（po/V）相同的原则为放大依据，将5L罐实验结果放大至30L小试及300L中试规模，30L罐发酵MTG酶活和MTG生产强度均高于5L罐，300L罐中MTG酶活达到3.15u/mL，MTG的平均比合成速率达到2.68uh^-1g^-1，接近于5L罐的MTG平均比合成速率，表明本研究所采用的通气量、搅拌转速等放大原则是可行的。

CONDITIONS FOR PILOT PRODUCTION OF MICROBIAL TRANSGLUTAMINASE (MTG)

The effect of starch pretreatment on microbial transglutaminase(MTG) production was investigated in a 5 L fermentor. The result showed that MTG activity, MTG productivity and MTG yield increased by 32%, 32% and 70%, respectively compared with the production from medium with unliquified starch. Gradual scale up principals were studied during the process of MTG pilot production. The production scale was enlarged from the 5 L fermentor to a 30 L and even a 300 L fermentor. The results showed that the scale up principles of the same aeration rate and the same agitated power per volume were obtained during the gradual scale up MTG production. When the above principles were applied, MTG activity and MTG productivity in 30 L fermentor were all higher than those in 5 L fermentor. MTG activity obtained 3.15 u/mL and average specific MTG formation rate reached 2.68 u h -1 g -1 in 300 L fermentor, which were almost equal to those in 5 L fermentor. Thus, it can be seen that the above principles are rational. Fig 3, Tab 2, Ref 12