| 酶催化光度法测定雨水中微量H_2O_2的研究 |
| |
| 引用本文： | 廖丽霞,宋丹丹,陈碧华,何阳.酶催化光度法测定雨水中微量H_2O_2的研究[J].环境科学与技术,2006,29(7):17-18. |
| |
| 作者姓名： | 廖丽霞 宋丹丹 陈碧华 何阳 |
| |
| 作者单位： | 华中师范大学化学学院,武汉,430079 |
| |
| 基金项目： | 湖北省自然科学基金重点攻关项目 |
| |
| 摘 要： | 研究了过氧化氢酶/4-氨基安替比林/苯酚/过氧化氢催化体系显色反应的最佳条件,实验发现:在温度为25℃,pH为7.0的条件下催化反应30min,所形成的催化反应产物在505nm处有最大吸收,H2O2在0.003～0.3000μmol/mL呈良好线性关系,其检测限达0.002μmol/mL(S/N=3),相对标准偏差RSD=0.24%(C=0.1000μmol/mL,n=10)。该法具有所用仪器简单,操作方便,灵敏度高等特点,应用于雨水中微量过氧化氢的测定,结果满意。
|
| 关 键 词： | 过氧化氢 4-氨基安替比林 分光光度法 |
| 文章编号： | 1003-6504(2006)07-0017-02 |
| 收稿时间： | 2005-09-19 |
| 修稿时间： | 2005-11-04 |
Catalytic Spectrophotometry with Enzyme for Determination of Hydrogen Peroxide in Rainwater |
| |
| LIAO Li-xia,SONG Dan-dan,CHEN Bi-hua,HE Yang.Catalytic Spectrophotometry with Enzyme for Determination of Hydrogen Peroxide in Rainwater[J].Environmental Science and Technology,2006,29(7):17-18. |
| |
| Authors: | LIAO Li-xia SONG Dan-dan CHEN Bi-hua HE Yang |
| |
| Abstract: | Reaction conditions of catalase/4-aminoanipyrine/phenol/hydrogen peroxide catalytic system were studied,and results showed at temperature of 25℃,pH 7.0,reacting time of 30 min,the product of catalytic system obtained maximum absorption at 505nm.The linear range for determination of H2O2 was found to be 0.003～0.3000μmol/mL with a detection limit of 0.002μmol/mL and relative standard deviation of 0.24%.The proposed method is simple,convenient and highly sensitive,which can be applied to direct determination of hydrogen peroxide in rainwater with satisfactory results. |
| |
| Keywords: | hydrogen peroxide 4-aminoantipyrine spectrophotometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
