Isolation and characterization of novel phorate-degrading bacterial species from agricultural soil |
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Authors: | Monu Jariyal V. K. Gupta Kousik Mandal Vikas Jindal Geetika Banta Balwinder Singh |
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Affiliation: | 1. Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India 2. Insect Molecular Biology Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India 3. Pesticide Residue Analysis Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India
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Abstract: | Based upon 16S rDNA sequence homology, 15 phorate-degrading bacteria isolated from sugarcane field soils by selective enrichment were identified to be different species of Bacillus, Pseudomonas, Brevibacterium, and Staphylococcus. Relative phorate degradation in a mineral salt medium containing phorate (50 μg ml?1) as sole carbon source established that all the bacterial species could actively degrade more than 97 % phorate during 21 days. Three of these species viz. Bacillus aerophilus strain IMBL 4.1, Brevibacterium frigoritolerans strain IMBL 2.1, and Pseudomonas fulva strain IMBL 5.1 were found to be most active phorate metabolizers, degrading more than 96 % phorate during 2 days and 100 % phorate during 13 days. Qualitative analysis of phorate residues by gas liquid chromatography revealed complete metabolization of phorate without detectable accumulation of any known phorate metabolites. Phorate degradation by these bacterial species did not follow the first-order kinetics except the P. fulva strain IMBL 5.1 with half-life period (t½) ranging between 0.40 and 5.47 days. |
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