Direct electrochemistry of glucose oxidase and biosensing for glucose based on DNA/chitosan film |
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| Authors: | Tingting Gu Yang Zhang Fei Deng Jing Zhang Yasushi Hasebe |
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| Institution: | 1. School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China;2. Department of Life Science & Green Chemistry, Saitama Institute of Technology, Fukaya, 369-0293, Japan;1. Université Lyon, Université de Claude Bernard Lyon 1, UMR 5180, 43 Bd. 11 Novembre 1918, Laboratoire des Sciences Analytiques (LSA), CPE, 69622, Villeurbanne, France;2. Université du Maine UMR CNRS 6613, Bd. Olivier Messiaen 72085 LE MANS Cedex9, France;3. CNR (Consiglio Nazionale Ricerche) Clinical Physiology Institute, Pisa, Italy;4. Centre Nacional of Microelectronic (IMB-CSIC) Campus UAB, 08193 Bellaterra, Barcelona, Spain;1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;2. School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, PR China;1. Chemical Engineering Department, Lakehead University, Thunder Bay, Ontario, Canada;2. Chemical Engineering Department, Faculty of Engineering, Alexandria University, Alexandria, Egypt;3. Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390;4. Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 |
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| Abstract: | Glucose oxidase (GOD) is widely used in the glucose biosensor industry. The amperometric biosensors based on directly electron transfer (DET) between an electrode and immobilized GOD are especially promising. In this article, GOD was immobilized with a DNA/chitosan bio-material film on GC electrode, and the DET of GOD on DNA/chitosan was studied. The cyclic voltammetric results indicated that the GOD immobilized in the DNA/chitosan film underwent DET reaction, and the cyclic voltammogram displayed a pair of well-defined redox peaks with a formal potential of ?0.45 V (vs. Ag/AgCl) at pH 5.5. The response showed a surface-controlled electrode process with an electron transfer rate constant of 0.91 sec?1 determined in the scan rate range from 10 to 100 mV/sec. The GOD immobilized in DNA/chitosan membrane retained its biocatalytic activity and stability. The immobilized GOD could electrocatalyze the reduction of dissolved oxygen and resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection with a sensitivity of 0.48 μA/(mmol/L), a linear range from 0.04 to 2.28 mmol/L and a detection limit of 0.04 mmol/L at a signal-to-noise ratio of 3. The sensor could exclude the interference of commonly coexisted uricacid and ascorbic acid. |
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