邻苯二甲酸二乙基己酯(DEHP)对小鼠的氧化损伤及黄豆异黄酮对其保护作用的研究

邻苯二甲酸二乙基己酯(DEHP)是环境中广泛存在的污染物,作为一种内分泌干扰物,已严重威胁到人类健康和生态环境安全。每天用250、500和1000mg·kg-1·d-1的DEHP对雄性昆明小鼠进行灌胃处理,连续处理30d后,分别考察了小鼠的器官相对质量、丙二醛(MDA)和谷胱甘肽(GSH)的含量以及睾丸组织学等指标的变化。结果显示,小鼠的肝、肺组织肿大,睾丸严重萎缩,并呈剂量依赖性。肝脏MDA含量显著增加,肝脏和血液GSH水平显著下降。从睾丸组织学分析结果可见,精子生长发育条件已受到破坏,例如睾丸细胞坏死和凋亡,生精小管上皮细胞大量脱落等。DEHP对未成熟的雄性小鼠有氧化损伤作用和生殖毒性,特别是在较高剂量(500and1000mg·kg-1·d-1)时。为了探讨大豆异黄酮(SI)对DEHP诱导的氧化损伤和睾丸损伤的保护作用,将暴露于1000mg·kg-1·d-1DEHP的小鼠,用100mg·kg-1·d-1剂量的SI平行处理。结果显示,SI作为抗氧化剂和雌激素调节剂可明显减弱DEHP所致的氧化应激和睾丸毒性。

Oxidative Damage Induced by Di-(2-ethylhexyl) Phthalate and Protective Effects of Soybean Isoflavones in Mice

Di-(2-ethylhexyl) phthalate (DEHP) is widespread in the environment. As endocrine disruptors, it has seriously threatened human health and ecological environment safety. Male Kunming mice were treated daily with DEHP at the dosages of 250, 500 and 1 000 mg·kg^-1·d^-1, the relative organ weight, the contents of malondialdehyde (MDA) and L-Glutathione (GSH), and testis histology were investigated after 30-d DEHP treatment. Liver and lung enlargement and severe testicular atrophy were observed in a dose-dependent manner. The contents of hepatic MDA significantly increased, while the levels of hepatic and blood GSH both significantly decreased. In testis histological analysis, disruptions of spermatogenesis were observed such as necrosis and apoptosis of testicular cells, and massive sloughing of seminiferous tubule epithelium. The results showed that DEHP caused oxidative damage and reproductive toxicity in immature male mice, particularly at relatively high doses (500 and 1 000 mg·kg^-1·d^-1). To investigate the protective effect of soybean isoflavones (SI) on DEHP-induced oxidative damage and testicular injury, the mice were exposed to 1 000 mg·kg^-1·d^-1 DEHP and 100 mg·kg^-1·d^-1 SI. It is demonstrated that SI supplementation could significantly attenuate DEHP-induced oxidative stress and testicular injury in mice, probably due to the properties of SI as antioxidant and estrogen regulator.