| 微囊藻毒素RR的制备及鉴定 |
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| 引用本文： | 陈晓国,肖邦定,徐小清.微囊藻毒素RR的制备及鉴定[J].中国环境科学,2005,25(3):0-0. |
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| 作者姓名： | 陈晓国 肖邦定 徐小清 |
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| 作者单位： | 1.武汉理工大学资源与环境工程学院 湖北武汉430070;2.中国科学院水生生物研究所 湖北武汉430070;3.中国科学院研究生院 北京100039 |
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| 基金项目： | 国家“973”项目(2002CB412300)，国家“863”项目(2001AA641030) |
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| 摘 要： | 以野外收集的水华蓝藻为原料,建立了以75%甲醇溶液提取、快速色谱和半制备色谱分离为主要步骤的微囊藻毒素分离纯化方法,并用HPLC、分光光度计和电喷雾质谱对所得毒素的纯度和结构进行了鉴定.结果表明,所得毒素为MCRR,纯度大于95%,其紫外吸收光谱在239nm处有特征吸收,分子组成为环(Ala-Arg-MeAsp-Arg-Adda-Glu-Mdha),分子量为1037.
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| 关 键 词： | 微囊藻毒素RR 纯化 鉴定 |
| 文章编号： | 1000-6923(2005)03-0267-04 |
| 收稿时间： | 1900-01-01; |
| 修稿时间： | 2004年10月29 |
Preparation and identification of microcystin-RR |
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| CHEN Xiao-guo,XIAO Bang-Ding,XU Xiao-qing.Preparation and identification of microcystin-RR[J].China Environmental Science,2005,25(3):0-0. |
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| Authors: | CHEN Xiao-guo XIAO Bang-Ding XU Xiao-qing |
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| Institution: | CHEN Xiao-guo1,XIAO Bang-ding2,3*,XU Xiao-qing2 |
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| Abstract: | Methods were established to isolate and purify microcystin with the main steps of extraction with 75% aqueous methanol, and isolation and purification with reversed-phase flash chromatography and semipreparative chromatography. The purity and structure of the obtained microcystin were identified with HPLC, spectrophotometer, and electrospray ionization mass spectrometry. The obtained microcystin was MCRR with purity >95% and its UV absorption spectra had characteristic absorption at 239nm. Its molecular composition was cyclo-(Ala-Arg-MeAsp-Arg-Adda-Glu-Mdha), and molecular weight was 1037. |
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| Keywords: | microcystin RR purification identification |
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