生物钯强化反硝化及群体感应调控

水体氮素污染对生态环境和人类健康具有严重的威胁，因此,探索高效的污水脱氮方法成为水处理领域高度关注的焦点。本研究利用厌氧活性污泥原位合成零价钯(Bio-Pd⁰)，探究了钯负载量对反硝化效果的影响。结果显示，负载适量的钯可以加快反硝化酶对电子的利用速率，从而减少亚硝酸盐和N₂O的积累，使硝酸盐的去除率提高了38.54%，而过量的钯负载会抑制生物反硝化效率。从微生物代谢和群体感应两方面进行了机理分析，结果表明，负载适量的钯能够促进微生物的代谢，从而提高对有机物的利用效率。并且添加0.2 g·L⁻¹群感抑制剂(QSI)猪肾酰化酶能进一步强化生物反硝化效果。本研究结果对改善污水脱氮效率具有重要的参考价值。

Biosynthetic palladium enhancing denitrification and quorum sensing regulation

Nitrogen pollution in waterbody posed a serious threat to eco-environment and human health. Therefore, to explore efficient denitrification methods has become a focus in the field of water treatment. In this study, zero-valent palladium (Bio-Pd0) was in-situ synthesized by anaerobic sludge, and the effect of palladium loading on denitrification was investigated. The results showed that an appropriate amount of palladium loading could accelerate the electron utilization efficiency, reduce the accumulation of nitrite and N2O, and increase the nitrate removal rate by 38.54%, while denitrification was inhibited with excessive palladium. The mechanism was analyzed from the aspects of microbial metabolism and quorum sensing. The results showed that an appropriate amount of palladium loading could promote the metabolic capacity of microorganisms and increase the organic utilization rate. Moreover, the addition of 0.2 g·L−1 group sensing inhibitor (QSI) porcine kidney acylase could further enhance the biological denitrification effect. The results of this study have an important reference for improving the denitrification effect of sewage.