| 粘红酵母(Rhodotorula glutinis CIBAS A 1401)苯丙氨酸解氨酶cDNA核心序列的克隆与分析 |
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| 引用本文： | 缪元颖,刘成君,杨顺楷,杨亚力.粘红酵母(Rhodotorula glutinis CIBAS A 1401)苯丙氨酸解氨酶cDNA核心序列的克隆与分析[J].应用与环境生物学报,2005,11(6):694-698. |
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| 作者姓名： | 缪元颖 刘成君 杨顺楷 杨亚力 |
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| 作者单位： | 1. 中国科学院成都生物研究所,成都,610041
2. 四川大学生命科学学院,成都,610064 |
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| 基金项目： | 浙江亚美生化股份有限公司资助，江苏常茂生物化学工程股份有限公司资助~~ |
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| 摘 要： | L苯丙氨酸因其在工业、医药上的重要作用而日益受到人们的重视.苯丙氨酸解氨酶(PAL)生物转化肉桂酸生产L苯丙氨酸成了该领域研究与开发的热点.本文利用简并寡核苷酸PCR法结合cDNA末端快速扩增PCR法,从粘红酵母中克隆了苯丙氨酸解氨酶cDNA核心序列,为克隆其cDNA全长序列及体外表达其活性蛋白奠定了基础.图5参22
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| 关 键 词： | 粘红酵母 苯丙氨酸解氨酶 基因克隆 cDNA |
| 收稿时间： | 2005-05-27 |
| 修稿时间： | 2005年5月27日 |
CORE cDNA CLONING AND STRUCTURAL ANALYSIS OF PHENYLALANINE AMMONIA-LYASE OF RHODOTORULA GLUTINIS CIBAS A 1401 |
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| MIAO Yuanying,LIU Chengjun,YANG Shunkai,YANG Yali.CORE cDNA CLONING AND STRUCTURAL ANALYSIS OF PHENYLALANINE AMMONIA-LYASE OF RHODOTORULA GLUTINIS CIBAS A 1401[J].Chinese Journal of Applied and Environmental Biology,2005,11(6):694-698. |
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| Authors: | MIAO Yuanying LIU Chengjun YANG Shunkai YANG Yali |
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| Abstract: | L-phenylalanine is now an important amino acid for food and medicine industries. The biotransformation of trans-cinnamic acid to L-phenylalanine by using phenylalanine ammonia-lyase (PAL) has, therefore, become very important in the research and development of this field. In this paper the gene encoding PAL was cloned by degenerating oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR procedure, and a core PAL cDNA sequence cloned was analyzed from Rhodotorula glutinis CIBAS A 1401. Fig 5, Ref 22 |
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| Keywords: | Rhodotorula glutinis CIBAS A 1401 phenylalanine ammonia lyase gene cloning cDNA |
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