| 水环境中溶解态腐殖酸对锌抗甲状腺激素干扰效应的影响 |
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| 引用本文： | 艾扬,孔东东,于畅,沈扬,李剑.水环境中溶解态腐殖酸对锌抗甲状腺激素干扰效应的影响[J].环境科学,2017,38(1):195-200. |
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| 作者姓名： | 艾扬 孔东东 于畅 沈扬 李剑 |
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| 作者单位： | 北京师范大学水科学研究院, 地下水污染控制与修复教育部工程研究中心, 北京 100875,北京师范大学水科学研究院, 地下水污染控制与修复教育部工程研究中心, 北京 100875,北京师范大学水科学研究院, 地下水污染控制与修复教育部工程研究中心, 北京 100875,北京师范大学水科学研究院, 地下水污染控制与修复教育部工程研究中心, 北京 100875,北京师范大学水科学研究院, 地下水污染控制与修复教育部工程研究中心, 北京 100875 |
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| 摘 要： | 本文以重金属锌为研究对象,应用重组人甲状腺激素受体(h TR)基因酵母快速测试方法,检测溶解态腐殖酸对锌抗甲状腺激素干扰效应的影响,利用阳极溶出伏安法(ASV)检测溶解态腐殖酸与锌作用后,生物有效态锌含量的变化,同时利用三维荧光光谱中的激发-发射矩阵(3DEEM)技术初步考察腐殖酸与锌的作用机制.结果表明,氯化锌具有显著的抗甲状腺激素干扰效应,其RIC20(抑制率为20%时氯化锌的浓度)值为1.70×10-5mol·L~(-1),当腐殖酸与氯化锌作用后,其抗甲状腺激素干扰效应降低了30%~50%,ASV测试结果表明当腐殖酸和氯化锌溶液混合后,生物有效态锌含量明显降低,与生物测试结果相似;紫外照射腐殖酸与氯化锌的混合体系后,其抗甲状腺激素干扰效应上升,但仍低于未加腐殖酸氯化锌溶液的抗甲状腺激素干扰效应值;3DEEM的测试结果表明氯化锌能够降低腐殖酸荧光峰的荧光强度,能够直观地表征腐殖酸与氯化锌相互作用.上述结果可为研究环境水体中锌的生物毒性及锌水质基准的制定提供基础数据和理论支持.
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| 关 键 词： | 溶解态腐殖酸 锌 甲状腺激素干扰效应 阳极溶出伏安法 三维荧光光谱 |
| 收稿时间： | 2016/5/27 0:00:00 |
| 修稿时间： | 2016/8/17 0:00:00 |
Effect of Dissolved Humic Acid on Thyroid Receptor Antagonistic Activity of Zinc in Aquatic Environment |
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| AI Yang,KONG Dong-dong,YU Chang,SHEN Yang and LI Jian.Effect of Dissolved Humic Acid on Thyroid Receptor Antagonistic Activity of Zinc in Aquatic Environment[J].Chinese Journal of Environmental Science,2017,38(1):195-200. |
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| Authors: | AI Yang KONG Dong-dong YU Chang SHEN Yang and LI Jian |
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| Institution: | Engineering Research Center of Ground Water Pollution Control and Remediation, Ministry of Education, College of Water Science, Beijing Normal University, Beijing 100875, China,Engineering Research Center of Ground Water Pollution Control and Remediation, Ministry of Education, College of Water Science, Beijing Normal University, Beijing 100875, China,Engineering Research Center of Ground Water Pollution Control and Remediation, Ministry of Education, College of Water Science, Beijing Normal University, Beijing 100875, China,Engineering Research Center of Ground Water Pollution Control and Remediation, Ministry of Education, College of Water Science, Beijing Normal University, Beijing 100875, China and Engineering Research Center of Ground Water Pollution Control and Remediation, Ministry of Education, College of Water Science, Beijing Normal University, Beijing 100875, China |
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| Abstract: | A rapid recombinant human thyroid (hTR) gene yeast bioassay was used to evaluate the effect of dissolved humic acid on thyroid receptor antagonistic activity of ZnCl2.The concentration of bio-available zinc after its reaction with dissolved humic acids was measured by anodic stripping voltammetry (ASV).Furthermore,the reaction mechanism of humic acid and zinc was investigated by three-dimensional excitation-emission matrix fluorescence spectroscopy (3DEEM).The results revealed that ZnCl2 demonstrated strong thyroid receptor antagonistic activity,and the concentration inhibiting 20% of the maximum effect of ZnCl2 was 1.70×10-5 mol·L-1.The thyroid receptor antagonistic activity of ZnCl2 was reduced by 30%-50% after the reaction of dissolved humic acids.The results of ASV showed that the concentration of bio-available zinc was decreased after the reaction of dissolved humic acids,the result was similar to that of bioassay test.The thyroid receptor antagonistic activity of the mixed solution of humic acid and ZnCl2 was increased after UV radiation treatment,however it was still lower than the antagonistic activity induced by ZnCl2.The results of 3DEEM showed that ZnCl2 could reduce the fluorescence peak intensity of humic acid,which could intuitively characterize the interaction between humic acid and ZnCl2.The above results can provide basic data and theoretical support for zinc toxicity study in aquatic environment and the establishment of water quality criteria for znic. |
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| Keywords: | dissolved humic acid zinc thyroid disrupting effects anodic stripping voltammetry 3DEEM |
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