4种反应器中厌氧氨氧化菌完整梯烷脂的特异性

完整梯烷脂是存在于厌氧氨氧化菌的厌氧氨氧化体膜上的一类特殊磷脂.分别从厌氧EGSB反应器、好氧SBR反应器、自养脱氮SBBR反应器、以及厌氧氨氧化EGSB反应器中提取了污泥样品中的磷脂混合物,利用高效液相色谱-电喷雾串联三重四级杆质谱法检测了污泥磷脂提取物中完整梯烷脂的种类,分析了完整梯烷脂的特异性,同时利用高通量测序技术测定了自养脱氮SBBR反应器和厌氧氨氧化EGSB反应器污泥中微生物的群落结构.完整梯烷脂的分析结果表明厌氧EGSB反应器和好氧SBR反应器的污泥磷脂提取物中未检测到完整梯烷脂,而自养脱氮SBBR反应器和厌氧氨氧化EGSB反应器的污泥磷脂提取物中检测到了完整梯烷脂.自养脱氮SBBR反应器的污泥磷脂提取物中检测到了5种完整梯烷脂,厌氧氨氧化EGSB反应器的污泥磷脂提取物中检测到了7种完整梯烷脂.高通量测序结果证实自养脱氮SBBR反应器含有Candidatus Brocadia属与Candidatus Jettenia属An AOB,厌氧氨氧化EGSB反应器含有Candidatus Kuenenia属与Candidatus Jettenia属An AOB.结果说明完整梯烷脂为厌氧氨氧化菌所特有的磷脂物质.

Specificity of Intact Ladderane Lipids in Anaerobic Ammonium Oxidizing Bacteria from Four Reactors

Intact ladderane lipids are a special kind of phospholipids in the anammoxosome membrane of AnAOB.Phospholipid organic mixture was extracted respectively from four sludge samples in anaerobic EGSB reactor,aerobic SBR reactor,autotrophic nitrogen removal SBBR reactor and anaerobic ammonium oxidation EGSB reactor.Phospholipid extracts of sludge were detected by the HPLC-ESI-MS/MS method to determine the types of intact ladderane lipids,and the specificity of intact ladderane lipids was also analyzed,at the same time,the bacterial community structure of autotrophic nitrogen removal SBBR reactor and anaerobic ammonium oxidation EGSB reactor was determined by high-throughput sequencing technology.Analytical results of intact ladderane lipids showed that intact ladderane lipids were not detected from anaerobic EGSB reactor and aerobic SBR reactor,and were detected from autotrophic nitrogen removal SBBR reactor and anaerobic ammonium oxidation EGSB reactor.Five kinds of intact ladderane lipids were detected in the phospholipid extract of sludge from autotrophic nitrogen removal SBBR reactor,and seven kinds of intact ladderane lipids were detected in the phospholipid extract of sludge from anaerobic ammonium oxidation EGSB reactor.The results of high-throughput sequencing confirmed that the autotrophic nitrogen SBBR reactor contained AnAOB of the genera Candidatus Brocadia and Candidatus Jettenia,and the anaerobic ammonium oxidation EGSB reactor contained AnAOB of the genera Candidatus Kuenenia and Candidatus Jettenia.These results illustrated that intact ladderane lipids were the peculiar phospholipids in AnAOB.