Alpha-fetoprotein and acetylcholinesterase activity in first-and early second-trimester amniotic fluid |
| |
Authors: | Finn Stener Jørgensen MD Karin Sundberg Anne Gitte Rasmussen Loft Jørgen Arends Bent Nørgaard-Pedersen |
| |
Institution: | 1. Department of Obstetrics and Gynaecology, Sections of Ultrasound, Denmark
Department of Clinical Biochemistry, Copenhagen, Denmark;2. Statens Seruminstitut, and Danish Institute for Clinical Epidemiology, Copenhagen, Denmark |
| |
Abstract: | Normal ranges of amniotic fluid alpha-fetoprotein (AFP) and acetylcholinesterase activity (AChE) are described for gestational weeks 11–14 using rocket gel immunoelectrophoresis for AFP quantitation and a monoclonal antibody (4F19) enzyme antigen immunoassay for AChE activity measurement. The normal ranges were established by the examination of 281 amniotic fluid samples from 281 normal pregnancies. AFP was found to increase from a median level of 14.0 MIU/1 at 11 weeks to a maximum at 13 weeks (median=18.0 MIU/l) (P<0.05), thereafter falling (not significant). No AChE test result exceeded 4.8 nkat/l. In addition, AFP and AChE values for three cases of fetal malformation, identified by the biochemical analyses of amniotic fluid, are given. These cases included two fetuses with a neural tube defect and one fetus with an abdominal wall defect. Amniocentesis was performed at 10, 11, and 14 weeks, respectively. The AFP and AChE values were all high. |
| |
Keywords: | alpha-fetoprotein acetylcholinesterase amniotic fluid normal ranges neural tube defect abdominal wall defect |
|
|