Identification of abnormal chromosomal complement in formalin-fixed,paraffin-embedded placental tissue |
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Authors: | Tracy A. Cowles MD Frederick F. B. Elder Suzanne Taylor |
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Affiliation: | Department of Pathology, The University of Texas, Houston, Health Science Center, Houston, Texas, U.S.A. |
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Abstract: | The objective of this project was to assess the efficacy of fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes to identify chromosome number in formalin-fixed, paraffin-embedded placental specimens. Using this approach, 75 per cent of the karyotypes in 20 formalin-fixed placental samples (comprising aneuploids, triploids, and normals) were correctly identified. As this technology improves, the ability to obtain information regarding chromosomal abnormalities in formalin-fixed, paraffin-embedded placental tissue should improve as well. This technology can potentially provide important cytogenetic information even when fresh tissue is not available for standard karyotypic analysis. |
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Keywords: | Fluorescence in situ hybridization (FISH) formalin-fixed placenta karyotype |
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