浑河底泥细菌与古菌群落结构空间变化特征研究

利用变性梯度凝胶电泳(PCR-DGGE)技术,考察了浑河表层底泥中细菌与古菌的多样性及群落结构变化情况.多样性结果表明,从上游至下游,底泥样品中细菌与古菌的种群结构均呈现由单一向复杂的演替趋势,且两者的Shannon多样性指数均在沈阳市下游的采样点-白塔堡河汇入浑河处分别达到其最大值3.88和2.91;而浑河底泥中细菌多样性及种群丰度均大于古菌多样性及种群丰度.群落结构的聚类分析结果表明,浑河底泥中细菌的群落结构具有明显区域特征,而古菌群落结构上下游保持稳定.冗余梯度分析(RDA)结果表明,上覆水中NO-3-N、NO-2-N、pH和Chl-a是影响细菌群落的主要因素,而NH+4-N、Chl-a、TP和NO-3-N则是影响古菌群落的主要因素.

Study on the spatial distributions of bacterial and archaeal communities in the sediments of Hun River

Denaturing Gradient Gel Electrophoresis (DGGE) was used to identify the diversities and spatial distributions of bacterial and archaea communities in the sediments of Hun River. Results show that both of the bacterial and archaeal community structures are relatively simple at upstream and more complex at downstream. The Shannon-Wiener Index of bacteria and archaea reached highest at 3.88 and 2.91 respectively, at the joint of Baitapu River and Hun River, located at the downstream of Shenyang city. The Shannon-Wiener Index and the abundance of bacteria are richer than that of archaea along the whole river. The communities of archaea in the sediments are consistent form the upstream to the downstream of Hun River, and the communities of bacteria are significantly different regionally. Results of RDA analysis show that the spatial distributions of the bacteria are greatly affected by four environmental indexes, i.e. the concentrations of nitrate, nitrite, chlorophyll a and pH, while the spatial distributions of archaea were affected by concentration of ammonium, nitrate, total nitrogen and chlorophyll a.