BAF中不同高度海绵铁填料表面物种多样性分析

主要通过目的片段的聚合酶链式反应(polymeraes chain reaction,PCR)扩增、Illumina高通量测序以及生物信息分析对曝气生物滤池(BAF)反应器中不同高度的海绵铁填料表面微生物丰度和多样性进行分析和研究。结果表明:Ctrl(原水样)以及反应器中优势种群均以变形菌门(Proteobacteria)为主,拟杆菌(Bacteroidetes)次之;等级丰度分布(Rank-Abundance)曲线中Ctrl物种数量为1 193,DO1(BAF上部水样)为2 389,DO2(BAF中部水样)为2 211,DO3(BAF下部水样)为1 890,DO1的丰度和生物多样性最高,随着DO浓度的降低(或填料空间位置的下降),微生物群落丰度和多样性均逐渐下降;Chao1指数比观察到的物种(observed species)指数对物种的丰度更加敏感,测得的OTUs(operational taxonomic units)数均高出400;DO1和DO2的物种均匀度较高,DO3和Ctrl的优势菌群所占比例很高,均匀度较低。通过PCoA(principal co-ordinates analysis)可以观察到DO1和DO2的物种组成最为相似。

Analysis of surface species diversity of sponge iron filler with different height in BAF

The microbial abundance and diversity of sponge iron filler in different height of BAF reactor were analyzed and studied by polymeraes chain reaction(PCR) amplification of the target fragment, Illumina high-throughput sequencing and biological information analysis. The results show that the dominant species in the raw Ctrl (raw water) and the reactor were mainly Proteobacteria, followed by Bacteroidetes. In Rank-Abundance distribution curve, the number of Ctrl species is 1 193, while that of DO1 (BAF upper), DO2 (BAF middle) and DO3 (BAF lower) is 2 389, 2 211 and 1 890, respectively, and DO1 has the highest abundance and species diversity. In the reactor, with the decrease of DO (or the decrease of filler spatial position), the richness and diversity of microbial community decreased gradually. It is found that the Chao1 index is more sensitive to the species richness than the observed species index, and the measured number of operational taxonomic units (OTUs) is about 400 higher. DO1 and DO2 samples had higher homogeneity, and the proportion of dominant bacteria in DO3 and Ctrl samples was high and the homogeneity was low. The species composition of DO1 and DO2 was observed to be most similar by PCoA.