| 壬基酚降解菌产酶条件的优化 |
| |
| 引用本文： | 张美珍,邓国志,张小凡,何义亮.壬基酚降解菌产酶条件的优化[J].环境污染与防治,2012,34(6):46-50. |
| |
| 作者姓名： | 张美珍 邓国志 张小凡 何义亮 |
| |
| 作者单位： | 1. 上海交通大学环境科学与工程学院,上海,200240
2. 上海交通大学环境科学与工程学院,上海200240;安徽大学资源与环境工程学院,安徽合肥230601 |
| |
| 基金项目： | 国家自然科学基金资助项目，"十一五"国家重大科技水专项 |
| |
| 摘 要： | 通过单因素试验和正交试验确定了实验室保藏的壬基酚降解菌株沙雷氏菌(Serratiasp.LJ)的最佳产酶条件:以壬基酚、硫酸铵为碳源、氮源,培养基初始pH为6.8,培养温度为30℃,种子活化时间为24h,接种量为3%(体积分数)。在此条件下培养72h后,最高酶活力为1.314IU/mL,是优化前的1.77倍。
|
| 关 键 词： | 壬基酚降解菌 酶活 产酶条件 |
Optimization of conditions on nonylphenol-degrading enzyme production |
| |
| ZHANG Meizhen
,
DENG Guozhi
,
ZHANG Xiaofan
,
HE Yiliang.Optimization of conditions on nonylphenol-degrading enzyme production[J].Environmental Pollution & Control,2012,34(6):46-50. |
| |
| Authors: | ZHANG Meizhen DENG Guozhi ZHANG Xiaofan HE Yiliang |
| |
| Institution: | 1.(1.School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240;2.College of Resource and Environmental Engineering,Anhui University,Hefei Anhui 230601) |
| |
| Abstract: | The optimal conditions of nonylphenol-degrading strains named Serratia sp.LJ for enzyme production were obtained by means of single-factor analysis and orthogonal design.Results showed that the optimal enzyme production conditions were applying nonylphenol,ammonium sulfate as carbon and nitrogen sources,cultured under initial pH of 6.8 at 30 ℃,gave the seed activation time of 24 h and inoculum of 3%.Under these optimal conditions for 72 h,the enzyme activity was up to 1.314 IU/mL,1.77 times as high as the original activity. |
| |
| Keywords: | nonylphenol-degrading strains enzyme activity conditions for enzyme production |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
