共基质体系下漆酶介体系统降解氮杂环化合物的研究

采用2,2-连氮-双-(3-乙基苯并噻吡咯啉-6-磺酸)(ABTS)作为介体,与纯漆酶组成漆酶介体系统(LMS),分别研究了单基质和共基质体系下LMS对氮杂环化合物的降解。结果表明:(1)LMS对单基质和共基质体系中的吲哚的降解反应很快达到平衡,约为1h。(2)共基质体系中吡啶的引入,对吲哚的降解没有明显的促进或抑制作用;共基质体系中喹啉的引入,对吲哚的降解有较小的促进作用,而同时引入吡啶和喹啉,一定程度上也促进了吲哚的降解。(3)喹啉不能被LMS所降解。共基质体系中吲哚的引入,对吡啶的降解有一定的抑制作用;对喹啉的降解有一定的促进作用。(4)在LMS的作用下,推断吲哚生成的中间产物是氧化吲哚和靛红;吡啶生成的产物是戊二醛和戊二酸;喹啉最先生成2-羟基喹啉。

Study on effects of co-substrates on the biodegradation of nitrogenous heterocyclic compounds by laccase mediator system

The laccase mediator system(LMS) was established by mixing pure laccase in 2,2-azino-bis(3-ethylbenz-thiazoline-6-sulfonate)(ABTS) mediator.The biodegradation of nitrogenous heterocyclic compounds by LMS under single substrates or co-substrate was researched.The results showed that indole could be degraded effectively by LMS under both single substrate systems and co-substrate systems,the degradation reached the equilibrium state rapidly,about 1 h.Adding pyridine in co-substrate had no obvious effect on indole degradation,adding quinoline in co-substrate would promote indole degradation slightly,while the degradation would be improved to a certain degree when adding pyridine and quinoline in co-substrate simultaneously.Quinoline could not be degraded by LMS,indole in co-substrate could inhibite pyridine degradation but promote quinoline degradation.With the help of HPLC,it could be concluded that the intermediate product of indole were oxindole and isatin,the intermediate product of pyridine were glutaric dialdehyde and glutarate,and quinoline was first produced to 2-hydroxy-quinoline.