温度, 光照和盐度对产麻痹性贝类毒素藻类生长及产毒的影响

为探究温度、光照和盐度对产麻痹性贝类毒素（PSP）藻类生长及产毒的影响，本文选取了4株产麻痹性贝毒的藻种（塔玛亚历山大藻（Alexandrium tamarense）香港株（ATHK）、微小亚历山大藻（A.minimum）台湾株（AMSY）、链状亚历山大藻（A.catenella）南海株（ACSY）和链状裸甲藻（Gymnodinium catenatum）防城巷株（GCFC））在不同光照（60、120和200 μmol/（m2·s））、温度（16、22和28℃）和盐度（25、30和35）条件下生长及产毒的变化。结果显示，22℃最适宜4株藻的生长。低温可促进PSP的产生，除GCFC外，其余3株藻的单位细胞产毒量均随温度的升高而降低。ATHK和AMSY的生长与光照正相关。AMSY和GCFC的单位藻细胞产毒量与光照强度正相关，光照为120 μmol/（m2·s）时ATHK和ACSY产毒量最高，弱光均不利于4种藻产毒。盐度30条件下ATHK，AMSY和GCFC长势较好，高盐度（盐度35）促进ACSY的生长。ATHK的单位细胞产毒量均随盐度的升高而增加，AMSY和ACSY在高盐和低盐条件下产毒量均较大，GCFC则在盐度为30条件下产毒量最高。

Effects of temperature,light intensity and salinity on growth and toxin production of paralytic shellfish poisoning toxins-producing dinoflagellates

Experiments were carried out to investigate the effects of light intensity(60, 120 and 200 μmol/(m2·s)), temperature(16, 22 and 28℃) and salinity(25, 30 and 35) on growth and paralytic shellfish poisoning(PSP) toxins production by Alexandrium tamarense HongKong strain(ATHK), A. minimum Taiwan strain(AMSY), A. catenella Nanhai strain(ACSY) and Gymnodinium catenatum Fangchenggang strain(GCFC). The results showed that 22℃ was the optimum growth temperature for all the four strains. Low temperature promoted PSP toxins production in all of the four strains. Except for GCFC, PSP toxins production in the other three strains were reduced with the temperature rising. The growth of ATHK and AMSY were positively correlated with light intensity. Toxin production in AMSY and GCFC were also positively correlated with light intensity. Toxicity of both ATHK and ACSY were also affected by light intensity, with maximum toxicity obtained at the light intensity of 120 μmol/(m2·s). Low light intensity inhibited toxin production in all of the four strains. ATHK, AMSY and GCFC all grew well at the salinity of 30, while ACSY grew well at the salinity of 35. Toxin production in ATHK increased with the increasing salinity. Both higher and lower salinities promoted the toxins production in AMSY and ACSY. A high level of toxin was detected in GCFC at the salinity of 30.