黄海桑沟湾养殖区海水中喹诺酮类抗生素的残留状况

渔业养殖水环境中抗生素污染造成的水产品质量安全和环境微生物耐药性问题已经引起广泛关注。本文采用固相萃取-液相色谱/串联质谱法（LC-MS/MS）对桑沟湾养殖区海水及养殖水产品中17种喹诺酮类抗生素药物残留进行研究。结果显示，养殖区海水中喹诺酮类抗生素的检出率高达52.94%，含量水平介于ND~32.48 ng/L。不同的鱼类养殖区域海水中喹诺酮类药物含量差别较大，且浓度大小依次为为牙鲆（Paralichthys olivaceus）养殖区>黑鲪（Sebastes schlegelii）养殖区>红鳍东方鲀（Takifugu rubripes）养殖区。科普示范养殖区海水中喹诺酮类最高浓度范围与牙鲆养殖区相近，而近岸码头非养殖区海水中喹诺酮类检出率和残留浓度最低，表明桑沟湾海水中抗生素残留可能受水产养殖的影响。分析结果显示桑沟湾养殖区鱼肉中喹诺酮残留量远小于国家安全限量。

Quinolone residues in seawater of aquaculture area,Sanggou bay,Yellow Sea,China

The pollution of antibiotics in water of aquaculture area, the quality and safety of aquatic products, as well as the problem of environmental microbial resistance, have been paid attention widely.In this paper, the concentration of 17 quinolones in seawater and aquaculture aquatic products from Sanggou bay was studied by solid phase extraction-liquid chromatography and tandem mass spectrometry (LC-MS/MS).The results showed that the positive rate of seawater samples contained quinolone residues was up to 52.94%, but the content was low, ranging from ND~32.48 ng/L.The quinolone contents in seawater samples from different fish aquiculture zones were variant largely.The highest quinolone distribution in seawater was located in Paralichthys olivaceus aquiculture zone and followed by Sebastes schlegelii aquiculture zone.The lowest quinolone concentration of seawater was detected in Takifugu rubripes aquiculture zone.The scientific aquiculture demonstration zone was closer to the Paralichthys olivaceus aquiculture zone, and the highest quinolone concentration range was the same as that of Paralichthys olivaceus aquiculture zone.The positive sample rate and concentration of quinolones in the seawater of coastal waters were the lowest.These indicated that the source of antibiotics in Sanggou bay water body might be affected by aquaculture action.The residue of quinolone residues in fishes which were cultured in this aquaculture zone is much less than the national quinolone max residue limit.