基于氨基酸碳稳定同位素的黄渤海刺参产地溯源的研究

本研究利用气相色谱-燃烧-同位素比值质谱仪（GC-C-IRMS）测定黄渤海区域不同地区（辽宁、山东）刺参氨基酸的相对含量及其碳稳定同位素组成特征，探讨以这些指标作为海参产地溯源的可行性。测定结果显示，脯氨酸（F=4.13）、亮氨酸（F=9.08）、酪氨酸（F=8.78）的相对含量在两地之间存在显著差异，通过主成分分析法对氨基酸相对含量做降维处理后未能将两地海参做出明显区分；辽宁刺参氨基酸的δ13C值范围是-24.54‰~-11.87‰，山东刺参氨基酸的δ13C值范围是-27.36‰~-16.42‰，二者之间多数氨基酸的δ13C值呈现显著差异，选取其中的两种氨基酸Tyr和Ser的δ13C值作为指标，可明显区分二者。结果表明氨基酸的碳稳定同位素组成相比于其相对含量是作为区分辽宁、山东刺参更准确的指标。相关结论能为氨基酸的稳定同位素分析技术应用于刺参产地溯源研究提供理论依据。

Research on the origin traceability of sea cucumber (Apostichopus japonicus) in Liaoning and Shandong based on the compound-specific carbon isotope analysis of amino acids

In this study, we measured the relative contents and stable carbon isotope compositions of amino acids(AAs) in Apostichopus japonicus from Liaoning and Shandong by GC-C-IRMS, and evaluated its applicability in establishing the origin traceability of Apostichopus japonicus.Results showed that, the relative content of Pro(F=4.13), Ile(F=9.08) and Tyr(F=8.78) revealed significant difference between those two locations, and AAs compositions combined with principal component analysis did not discriminate samples of those two locations; the δ13C values of A. japonicus from Liaoning ranged from -24.54‰~-11.87‰ and the δ13C values of A. japonicus from Shandong ranged from -27.36‰~-16.42‰, most of the AAs δ13C values revealed significant difference between those two locations, we selected two AAs:Tyr and Ser, and their δ13C values could clearly discriminate samples of those two locations.So the stable carbon isotope composition of AAs is more precise than the AAs compositions for discriminating the samples of A. japonicus between Liaoning and Shandong.Thus, compound-specific carbon isotope analysis of AAs will provide a theoretical basis for establishing the traceability of the geographical origins of A.japonicus.