菌株Enterococcus sp.Y025利用海带水解液生长及产絮凝性质分析

利用盐酸和硫酸分别对海带进行预处理，通过测定还原糖得率及葡萄糖含量，分析海带水解效率。结果表明，盐酸浓度为1%时，海带还原糖得率为24.16%，葡萄糖含量达到0.32 mg/mL，海带水解效率较高。在不添加K₂HPO₄和KH₂PO₄等磷酸盐时，产絮凝菌株Y025（Genbank号为:KX950932）在不同海带水解液体积比（20%、40%、60%、80%和100%）培养条件下，都能够生长并具有产絮凝性质。海带水解液体积比为80%时，菌株Y025具有最高产絮凝率（52.91%）。菌株Enterococcus sp.Y025能够利用海带水解液生长并具有产絮凝性质，这为探究生物絮凝剂的规模化应用提供理论依据。

Characterization of cell growth and bioflocculant production by Enterococcus sp.Y025 using macro-algae Laminaria japonica

In this study, the different acid pretreatments(with HCl and H₂SO₄) were applied to compare the sacchariflcation efficiency of Laminaria japonica which including the total reducing sugars and glucose. According to the two indicators, the results showed that the sacchariflcation efficiency from L. japonica that was pretreated with 1% HCl was the highest, with the total reducing sugars 24.16% and glucose concentration 0.32 mg/mL. A new bioflocculant producing bacterium Enterococcus sp. Y025(Genbank: KX950932) could grow and produce bioflocculant using acid pretreated L. japonica as the optimal substrate without the addition of K₂HPO₄ and KH₂PO₄. The cell growth and bioflocculant production of strain Y025 under different volume ratio of hydrolysate(20%, 40%, 60%, 80%, and 100%) were investigated. The result indicated that the optimal volume ratio of hydrolysate was 80%, with high bioflocculant production of 52.91%. Macro-algae such as L. japonica show the prospect of high biomass yields. The result indicated that the strain Y025 has the ability to produce bioflocculant using the acid pretreated L. japonica, which is beneficial for the improvement of cost-effective bioflocculant production.