| 模拟酶蛋白结合物替代HEMIN测定大气过氧化物 |
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| 引用本文： | 马一琳,张远航,曾立民.模拟酶蛋白结合物替代HEMIN测定大气过氧化物[J].环境化学,2000,19(5):460-465. |
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| 作者姓名： | 马一琳 张远航 曾立民 |
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| 作者单位： | 环境模拟与污染控制国家联合重点实验室,北京大学环境科学中心,北京,100871 |
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| 基金项目： | 教育部跨世纪优秀人才培养计划，， |
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| 摘 要： | 在选择最佳测定条件的基础上，对比了模拟酶蛋白结合物和ＨＥＭＩＮ对过氧化物的测定效果。结果表明，ＨＥＭＩＮ－ＢＳＡ效果最好，其对过氧化物的荧光响应信号约是ＨＥＭＩＮ的２倍，该物质在不改变激发和发射光谱的前提下可增强反应活性，提高反应灵敏度，使方法检测限降低至使用ＨＥＭＩＮ时的１／３左右，最低线性值降至原来的１／５左右，同时，精度亦有所提高。
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| 关 键 词： | 模拟 酶蛋白结合物 过氧化物 HEMIN 大气监测 |
MIMIC ENZYME-PROTEIN COMBINATION AS THE SUBSTITUTE OF HEMIN IN THE DETECTION OF ATMOSPHERIC PEROXIDES |
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| Ma Yilin,Zhang Yuanhang,Zeng Limin.MIMIC ENZYME-PROTEIN COMBINATION AS THE SUBSTITUTE OF HEMIN IN THE DETECTION OF ATMOSPHERIC PEROXIDES[J].Environmental Chemistry,2000,19(5):460-465. |
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| Authors: | Ma Yilin Zhang Yuanhang Zeng Limin |
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| Abstract: | Based on choosing the best test condition, the detecting effect of peroxides by mimic enzyme-protein combination and HEMIN are compared. HEMIN-BSA has the best effect, which fluroescent respones is as twice high as that of HEMIN. The detection limit of the method is 1/3 of HEMIN method, the minimum linear limit is 1/5 of that of HEMIN and it has better relative standard deviation also. |
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| Keywords: | Mimic enzyme-protein combination Peroxides HEMIN Bovine serum albumin(BSA) |
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