Fenton氧化—微生物法降解土壤中石油烃

以长期被苯系物污染的活性污泥为菌源,采用液相“诱导物-中间产物-目标污染物”驯化模式驯化出专性混合石油降解菌群,并将其用于Fenton氧化—微生物法处理模拟石油污染土壤。高通量测序结果表明,产黄杆菌属（Rhodanobacter）、分支杆菌属（Mycobacterium）和根瘤菌属（Rhizobiales）为主导菌属。实验结果表明：接种混合菌群后降解50 d,土样的总石油烃（TPH）去除率较土著菌提高了13.4~20.5百分点;对于TPH含量（w）分别为4%,8%,11%的土样,Fenton氧化的最佳H₂O₂加入量分别为3,4,4 mol/L（Fe²⁺加入量0.04 mol/L）,TPH总去除率分别可达88.8%,65.0%,47.7%,较单独Fenton氧化或单独微生物法均有很大程度的提高,且缩短了降解时间,增加了土壤有机质。

Degradation of petroleum hydrocarbons in soil by Fenton oxidation-microbial method

Using the activated sludge which was long-term polluted by benzene as bacteria source,the specific mixed petroleum-degrading bacteria were acclimated in liquid phase by the model of “inducer-intermediate product-target pollutant”,and used for treatment of simulated petroleum contaminated soil by Fenton oxidation-microbial method. The high-throughput sequencing results showed that Rhodanobacter sp.,Mycobacterium sp. and Rhizobiales sp. were the dominant bacteria. The experimental results indicated that：After 50 d of degradation,the removal rate of total petroleum hydrocarbon（TPH）in the soil samples by mixed bacteria was 13.4-20.5 percentage points higher than that by native bacteria;When the TPH content（w）were 4%,8%,11%,the optimum H₂O₂ concentration for Fenton oxidation were 3,4,4 mol/L respectively（with 0.04 mol/L of Fe²⁺ concentration）,the total removal rate of TPH were 88.8%,65.0%,47.7% respectively,which were much higher than those by single Fenton oxidation or single microbial method. The degradation time was shortened and organic matters in soil were increased by the combined process.