Monitoring potential semiochemicals in individual mouse urine samples |
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Authors: | M N Kayali-Sayadi L M Polo-Diez I Salazar |
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Institution: | (1) Mass Spectrometry Service, Faculty of Chemistry, Universidad Complutense de Madrid, E-28040 Madrid, Spain;(2) Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, E-28040 Madrid, Spain;(3) Department of Anatomy and Embryology, Faculty of Veterinary Science, , E-27002 Lugo, Spain |
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Abstract: | Summary. This work validates a method for detecting potential semiochemicals in mouse urine samples with a volume as small as 10 μL. Using solid-phase microextraction, gas chromatography and mass spectrometry, we screened 2,3,5-trithiahexane, 2-sec-butyl-4,5-dihydrothiazole, geraniol, indole, trans-β-farnesene and farnesol in individual urine samples taken daily from mice housed under various social conditions. Excretion of 2-sec-butyl-4,5-dihydrothiazole by males did not occur when they were housed in rooms containing no females, but increased when raising the males to rooms containing females. Between-male differences in β-farnesene excretion were observed even in the absence of females. These results highlight the usefulness of the proposed analytical method for research in this area. |
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Keywords: | Metabolites mouse urine gas chromatography |
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