Superoxide dismutase in the marine sponge <Emphasis Type="Italic">Cliona celata</Emphasis> |
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| Authors: | Daniela Marques Ana I Esteves Marise Almeida Joana Xavier Madalena Humanes |
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| Institution: | 1.Departamento de Química e Bioquímica, Centro de Quimica e Bioquimica,Faculdade de Ciências da Universidade de Lisboa,Lisbon,Portugal;2.Instituto de Tecnologia Biomédica,Faculdade de Medicina Dentária da Universidade de Lisboa,Lisbon,Portugal;3.Institute for Biodiversity and Ecosystem Dynamics (IBED) and Zoological Museum of Amsterdam (ZMA),University of Amsterdam,Amsterdam,The Netherlands;4.CIBIO—Pólo A?ores, Centro de Investiga??o em Biodiversidade e Recursos Genéticos, e Departamento de Biologia,Universidade dos A?ores,Ponta Delgada,Portugal |
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| Abstract: | The aim of this work is to investigate the activity of the antioxidant enzyme superoxide dismutase in the cosmopolitan sponge
Cliona celata (Grant, 1826), since this enzyme has been described as a useful biomarker for marine pollution in other marine invertebrates.
The quantification of the catalytic activity for superoxide dismutase is quite complex because its substrate is an unstable
free radical. Several methods have been developed for this enzymatic activity determination; most of them are based on inhibition
of a redox reaction involving the superoxide radical. In this work, two methods are compared, for crude sponge extracts, as
far as repeatability, reproducibility and sensibility are concerned. The adrenaline oxidation method seems to be the most
adequate for these determinations. Statistical treatment of the data indicates that the reference value for the specific superoxide
dismutase activity in C. celata should be in the interval 0–535.5] U/mg of total protein in wet tissues, for normal populations. |
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