Quantification of Polycyclic Aromatic Hydrocarbons in Tea and Coffee Samples of Mumbai City (India) by High Performance Liquid Chromatography |
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Authors: | Narsi R. Bishnoi Urvashi Mehta Umashanker Sain G. G. Pandit |
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Affiliation: | (1) Department of Environmental Science & Engineering, Guru Jambheshwar University, Hisar, 125 001, India;(2) Environmental Assessment Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India |
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Abstract: | This paper describes a method for quantification of sixteen polycyclic aromatic hydrocarbons (PAHs) in tea and coffee samples of Mumbai City with the help of reversed phase high performance liquid chromatography with UV-VIS detector. This method is based on liquid–liquid extraction followed by clean up with C-18 cartridge. Concentration of total PAHs in different brands of tea and coffee samples varied from 18.79 to 31.37 μg/L and from 16.47 to 18.24 μg/L, respectively. Mean concentration of total PAHs was 27.56 μg/L in tea and 17.20 μg/L in coffee. Recoveries at different concentration levels were higher than 68% in samples of tea and coffee. Detection limit was found to be low (0.0006 ng) for anthracene and highest (0.174 ng) for naphthalene with relative standard deviation between 0.4%–7%. |
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Keywords: | coffee extraction hexane high performance liquid chromatography polycyclic aromatic hydrocarbon tea |
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