辽东湾潜在褐潮生物时空分布及环境关联

以18S rDNA V4区作为目标基因，利用自行设计的真核浮游植物鉴定引物V4（F/R），结合高通量测序技术，对辽东湾2014年四季海水中真核浮游植物多样性进行了检测.结果发现了8种潜在褐潮生物：抑食金球藻、金牛微球藻、微拟球藻、颗粒微拟球藻、细小微胞藻、密球藻、普通小球藻、钙质角毛藻，其中前2种生态风险较高，主要分布在辽东湾西南、东南两侧.除抑食金球藻和细小微胞藻为混合营养型外，其他6种均为自养型.细小微胞藻丰度最高（平均161445个/L），主要分布在秋季；其次是抑食金球藻、金牛微球藻（平均13912，13717个/L），主要分布在春季；之后为钙质角毛藻、普通小球藻（平均5498，5234个/L），主要分布在秋季；密球藻（平均1345个/L），主要分布在夏季；微拟球藻、颗粒微拟球藻最少（平均56，44个/L），冬季无分布.辽东湾潜在褐潮生物丰度与水温、水深、无机氮、N/P显著相关，其群落结构演替与环境因子关联性有待于进一步研究.

The temporal and spatial distribution of potential brown tide species and correlation analysis with environmental factors in Liaodong Bay

To gain an improved understanding of the mechanisms and species that drive brown tides, we used the V4region of 18S rDNA as the target gene to design the V4(F/R) primer and evaluated the diversity of the composition and relative abundances of the eukaryotic phytoplankton community in Liaodong Bay in different seasons with high-throughput sequencing technology. We found that some of the species in Liaodong Bay, such as Aureococcus anophagefferens, Ostreococcus tauri, Nannochloris sp., Nannochloropsis granulata, Micromonas pusilla, Pycnococcus provasolii, Chlorella vulgaris, and Chaetoceros calcitrans., had the potential to cause brown tides. Of these species, the risks were higher from Aureococcus anophagefferens and Nannochloropsis gaditana, which are distributed in the southwest and southeast of the bay, respectively. Aureococcus anophagefferens and Micromonas pusilla live with mixotrophs and other six potential brown tide species live with autotrophs.The abundance of Micromonas pusilla was the largest, with an average of 161445 ind/L, mainly occurring in autumn. Aureococcus anophagefferens and Ostreococcus tauri, with average abundances of 13912 and 13717 ind/L over the four seasons,respectively, mainly occurring in spring. Chlorella vulgaris and Chaetoceros calcitrans, with average abundances of 5498 and 5234 ind/L, respectively, were most abundant in autumn. Nannochloropsis gaditana. and Nannochloropsis granulata were absent in the winter and were present at only low abundances in other seasons. The correlations between the abundances of the brown tide species and environmental factors, such as the water temperature, water depth, N/P ratio, and inorganic nitrogen content, were significant. And the relationship between community structure succession and environmental factors need to be studied further.