ABR启动期运行效能及互营产甲烷菌群的分布特征

为探讨厌氧折流板反应器（ABR）启动期运行效能和互营产甲烷菌群的空间分布特征，考察了ABR反应器处理制糖废水启动期的运行特征，并采用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）技术分析了互营产甲烷菌群在ABR各格室的分布规律.结果表明，在污泥驯化阶段，ABR的COD去除率为61.5%，出水挥发酸总量高达1808mg/L.经过2个阶段的调控运行后，ABR出水挥发酸明显降低，甲烷含量增加至55%以上，COD去除率达到了94.8%.而且ABR第2~4格室形成了沉降性能良好的颗粒污泥.PCR-DGGE检测结果表明，该ABR系统中的主要产氢产乙酸菌为Syntrophobacter和Pelotomaculum，主要分布在ABR系统第3，4格室.ABR第1，2格室的产甲烷菌主要为耐酸的氢营养型产甲烷菌（Methanoregula和Methanosphaerula），而乙酸营养型产甲烷菌（Methanosaeta和Methanothrix）主要分布在第3，4格室.ABR系统中产甲烷菌的多样性要明显高于产氢产乙酸菌，说明当系统受到冲击时，产氢产乙酸作用比产甲烷作用更易成为限速步骤.

The performance of ABR at startup and the distribution of syntrophic methanogens

An anaerobic baffled reactor (ABR) was employed to investigate the operational performance and the distribution of syntrophic methanogens during the treatment of sugar refinery wastewater. The distribution of syntrophic methanogens was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). COD removal was 61.5% and volatile fatty acids (VFAs) in effluent were 1808mg/L at sludge acclimation stage. After the regulation operation of two stages, the VFAs were dramatically reduced and methane content was increased to above 55%. The COD removal was achieved 94.8% at the stable state. The granular sludge with good settling performance was formed in the last 3compartments. PCR-DGGE explored that the dominant hydrogen-producing acetogens were related to the genera Syntrophobacter and Pelotomaculum, which were distributed in the third and fourth compartments of ABR. The acid-tolerant hydrogenotrophic methanogens (Methanoregula and Methanosphaerula) were mainly distributed in the first and second compartments, while acetotrophic methanogens (Methanosaeta and Methanothrix) mainly existed in the third and fourth compartments. The present study found the diversity of methanogens was higher than that of hydrogen-producing acetogens, indicating hydrogen-producing acetogenesis is more likely to be a rate-limiting step when the system is shocked.