Pentachlorophenol degradation by Pseudomonas stutzeri CL7 in thesecondary sludge of pulp and paper mill |
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Authors: | Santosh Kr. Karn S. K. Chakrabarty M. S. Reddy |
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Affiliation: | 1. Department of Biotechnology,Thapar University,Patiala 147004,India 2. Thapar Centre for Industrial Research and Development,Yamunanagar 135001,India |
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Abstract: | A pentachlorophenol (PCP) mineralizing bacterium was isolated from the secondary sludge of pulp and paper mill and identifiedas Pseudomonas stutzeri strain CL7. This isolate used PCP as its sole source of carbon and energy and was capable of degrading thiscompound as indicated by stoichiometric release of chloride and biomass formation. P. stutzeri (CL7) was able to mineralize a highconcentration of PCP (600 mg/L) than any previously reported Pseudomonad with PCP as sole carbon source. As the concentrationof PCP increased from 50 to 600 mg/L, the reduction in the cell growth was observed and the PCP degradation was more than 90%in all studied concentrations. This isolate was able to remove 66.8% of PCP from the secondary sludge of pulp and paper mill whensupplemented with 100 mg/L of PCP and grown for two weeks. This study showed that the removal e ciency of PCP by CL7 wasfound to be very e ective and can be used in PCP remediation of pulp paper mill waste in the environment. |
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Keywords: | pentachlorophenol 16S rRNA secondary sludge bioremediation pulp and paper mill |
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