多基质时厌氧氨氧化菌、异养反硝化污泥活性及抑制特征

以厌氧氨氧化污泥(AAOB)和各种有机物混合,AAOB分别与异养甲醇反硝化菌和城市污水处理厂活性污泥相混合为研究对象,考察了多种基质(醇类、糖类)时自养、异养反硝化菌群的活性及其相应的抑制特征.结果表明,醇类有机物对AAOB的活性有明显抑制作用,甲醇的抑制性最强,当甲醇为5.48mmol/L时,AAOB活性了损失2/3.而乙酸钠对AAOB有一定的促进作用,另外,葡萄糖、乳糖和蔗糖等糖类有机物对AAOB的影响较小.此外,试验发现经驯化的甲醇反硝化菌能利用各种醇类完成异养短程反硝化.对于活性污泥,利用乙酸钠实现短程反硝化的能力优于甲醇、乙醇、葡萄糖和乳糖.混合试验中,正丙醇导致AAOB和甲醇反硝化菌混合菌群中AAOB活性下降,并且在与甲醇反硝化菌的竞争中处于劣势.乙酸钠对于AAOB和活性污泥混合菌群中AAOB的影响较小.

Activity and inhibition characteristics of anammox and heterotrophic denitrifier bacteria in a multi-substrate system

Consortiums from anammox, methanol denitrification process, and domestic wastewater treatment plant were used for nitrogen removal in a multi-substrate system by batch tests. The activity and inhibition characteristics of bacteria in denitrification were studied seriously. Experimental results showed that alcohols inhibited anaerobic ammonium oxidation bacterium, and the most serious inhibition came from methanol, leading to activity lose about 2/3 at concentrations of 5.48mmol/L. Sodium acetate strengthened AAOB activity, meanwhile, glucose, lactose and sucrose had no effect on its activity. These experimental results clearly indicated that the best hetero-denitrification carbon sources for methanol denitrifiers and activity sludge were methanol and sodium acetate respectively. In mixture consortium, inhibitor propanol made the AAOB at a disadvantage in the competition with the methanol denitrifiers, on the other side, the effect from sodium acetate to AAOB can be ignored in the competition with activity sludge.