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小麦籽实中蛋白质-Cd,Pb结合形态及其稳定性
引用本文:何孟常,杨居荣.小麦籽实中蛋白质-Cd,Pb结合形态及其稳定性[J].环境化学,2001,20(2):124-128.
作者姓名:何孟常  杨居荣
作者单位:北京师范大学环境科学研究所,
基金项目:国家自然科学基金资助项目(49771069)
摘    要:在体外消化酶模拟条件下,对小麦籽实中Cd,Pb蛋白质结合体的稳定性进行了研究.结果表明:小麦籽实中存在的表观分子量为54.5×103和5.5×103的蛋白质-Cd,Pb结合体,在体外胃蛋白酶和胰蛋白酶消化后,蛋白质结构发生变化.特别是分子量为54.5×103的Cd,Pb-蛋白质结合体不稳定,容易被胰蛋白酶分解,变成分子量为5.5×103的稳定的Cd,Pb-蛋白质结合体或更小的多肽.

关 键 词:小麦籽实  谷物  重金属      蛋白质  结合形态  稳定性  毒性  消化酶

THE PROTEIN-BINDING FORMS OF CADMIUM AND LEAD IN POLLUTED WHEAT SEED AND ITS STABILITY
HE Mengchang,YANG Jurong.THE PROTEIN-BINDING FORMS OF CADMIUM AND LEAD IN POLLUTED WHEAT SEED AND ITS STABILITY[J].Environmental Chemistry,2001,20(2):124-128.
Authors:HE Mengchang  YANG Jurong
Abstract:The stability of protein-binding forms of Cd and Pb in polluted wheat seed was evaluated using the treatment of gastrointestinal enzyme digestion.The results showed that after the treatment of pepsin and trypsin enzyme digestion,the protein-bin-ding forms of Cd and Pb with molecular weight of 54.5×103 and 5.5×103 in wheat seed was decomposed.Especially,the protein-binding forms of Cd and Pb with the apparent molecular weight of 54.5×103 were easily decomposed by the trypsin and changed into the low molecular weight protein-binding forms (5.5×103)or tiny peptide chain.
Keywords:wheat seed  protein - binding forms of heavy metals  stability  digestive en-zyme  
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