长三角地区MBBR泥膜复合污水厂低温季节微生物多样性分析

为表征长三角地区采用MBBR泥膜复合工艺（hybrid-MBBR）的污水厂在低温季节的微生物群落变化，得出微生物分布规律，采用Illumina MiSeq高通量测序对该区域5座市政污水处理厂进行研究，对好氧区活性污泥及悬浮载体生物膜微生物群落结构进行了分析.结果表明，同污水厂悬浮载体生物膜微生物物种数低于同系统活性污泥，且物种分布更不均匀.悬浮载体的投加可提升系统微生物多样性，但同时进水及运行方式对系统微生物群落组成具有一定选择性.各污水厂相对丰度较高的菌属主要有Nitrospira、Mycobacterium、Defluviicoccus、Hyphomicrobium和Macellibacteroides等，悬浮载体的投加极大程度上强化了优势硝化菌属Nitrospira的富集.核算悬浮载体中硝化细菌生物量占系统中总量的86.12%~95.36%；各污水厂好氧区悬浮载体中均检测到一定相对丰度的反硝化菌群，结合沿程及小试结果确认好氧区悬浮载体生物膜上发生了显著的同步硝化反硝化（SND）现象，强化了系统TN去除.

Microbial Diversity Analysis of WWTPs Based on Hybrid-MBBR Process in a Low Temperature Season in the Yangtze River Delta

The aim of this study was to characterize the changes of the microbial community in WWTPs based on hybrid-MBBR process in the Yangtze River Delta in a low temperature season, and to obtain the regularity of the microbial distribution. High-throughput sequencing of Illumina miSeq was conducted to analyze the microbial community structure of activated sludge and suspended carrier biofilm in the aerobic area of five WWTPs. The results showed that the number of microbial species in the suspended carrier biofilm was lower than that of the activated sludge in the same plant, and the species distribution was more uneven. The addition of a suspended carrier can improve the microbial diversity of the system, while the influent and operation mode have a certain selectivity to the microbial community composition of the system. The bacteria with high relative abundance in each plant primarily included Nitrospira, Mycobacterium, Defluvicoccus, Hyphomacrobium, and Macrocharacters,etc. The addition of suspended carriers significantly enhanced the enrichment of Nitrospira. The amount of nitrifying bacteria in the suspended carrier accounted for 86.12%-95.36% of that of the whole system. A certain relative abundance of denitrifying bacteria was detected in the suspended carrier in the aerobic area of each plant. Combined with the results of the measurement of water quality along the process and the lab-scale experiment, it was confirmed that significant SND occurred on the suspended carrier biofilm in the aerobic area, which enhanced the TN removal of the system.