污水稳定塘菌-藻生态系统去除与灭活植物病毒TMV研究

了解污水稳定塘生态系统去除与灭活植物病毒的效率与机理具有重要的理论和实际意义.对模型植物病毒———烟草花叶病毒(tobaccomosaicvirus,TMV)的实验结果显示:稳定塘系统中的悬浮固体(SS)可在短时间内吸附TMV且达到一个饱和值.纯培养的枯草芽孢杆菌及小球藻对TMV的存活无明显不良影响,而光合细菌和稳定塘中的混合细菌群却对TMV具有灭活作用.当它们同藻类形成菌藻共生体系时,对TMV的灭活速率分别从对照的每天0 0404log10(枯斑数·mL-1)升高到0 0783log10(枯斑数·mL-1)和0 075log10(枯斑数·mL-1) 因此,在污水稳定塘生态系统中,由于病毒对悬浮固体的吸附以及菌藻共生体系中微生物的作用可有效地去除与灭活植物病毒,其中悬浮固体吸附病毒并非真正意义上的灭活,稳定塘中存在的细菌特别是光合细菌对植物病毒的灭活才是最主要的因素.

Removal and inactivation of TMV by bacteria-algal ecosystem in a wastewater stabilization pond

The results of removal and inactivation of tobacco mosaic virus (TMV),a representative vegetal virus , in laboratorial models by algal-bacteria ecosystem in a sewage stabilization pond were studied. The results showed that: TMV can be rapidly adsorbed to the suspend solid in stabilization pond(SS)by a reversible process. The proportion of adsorption TMV was determined by the characteristics of the SS, and there was a saturation value of the adsorption TMV with every SS unit. The results of the microorganisms, which were found mostly in the bacteria-algal system, showed: The pure cultures of Bacillus subtitles and Chlorella vulgarism in sterile sewage had no effect on TMV survival, whereas, the growth of photosynthetic bacteria (PSB) and heterogeneous populations of stabilization pond bacteria (HSPB) in the same medium resulted in substantial virus inactivation. The ability of PSB and HSPB for the inactivation of TMV would be promoted after which they were cultured with algal and formed the bacteria-algal symbiosis system. The activity of PSB and HSPB was the most important factor for the inactivation of TMV in the sewage stabilization pond systems.