我国典型土壤对病毒等温静态吸附的数值模拟

通过室内等温静态批量平衡吸附实验,采用3种常见的等温线（Freundlich方程、Langmuir方程和Temkin方程）对2种病毒（MS2和X174）在2种处理（非灭菌和灭菌）条件下的6种土壤（红壤土、红粘土、乌栅土、黄泥土、沙质潮土和壤质潮土）中的吸附行为进行了回归拟合.实验结果和模拟结果均表明,土壤性质、病毒性质、土壤中的土著微生物对病毒在土壤中吸附行为均具有重要的影响.红粘土对MS2和X174的平均吸附比例几乎能达到100％,而2种潮土（沙质潮土和壤质潮土）相对较弱;总体来看土壤对X174的吸附能力高于MS2,但灭菌后的土壤对MS2的吸附能力却高于X174.在数值模拟中,Freundlich方程和Langmuir方程均具有理想的相关性.Freundlich方程能够表现出病毒浓度对其在土壤中吸附行为的影响;尽管Langmuir方程能够应用于土壤对病毒吸附能力的比较,但本研究中不能应用Langmuir方程来计算土壤对病毒的最大吸附量.

Numerical Simulation of Isothermal Equilibrium Adsorption of Virus onto Typical Soils in China

An isothermal batch experiment was conducted in the laboratory to compare adsorption of bacteriophages MS2 and phiX174 onto 6 different soils (red loam soil, red clay soil, wushan soil, huangni soil, sandy fluvo-aquic soil and loamy fluvo-aquic soil) in China. Soils with sterilized or non-sterilized treatment were used. Relative coefficients of each numerical simulation of the isotherms using three models were evaluated. The results show that the properties of the soil and virus, and presence/absence of the soil autochthonous microorganisms have pronounced effect on the virus adsorption. Both MS2 and phiX174 are almost completely adsorbed by the red clay soil, but minimal adsorption is observed in the two fluvo-aquic soils. Adsorption of phiX174 to all the non-sterilized soils is generally much greater than that of MS2, while sterilization leads to opposite results. Freundlich and Langmuir isotherms are found to have better coefficients to simulate the apparent steady-state virus concentrations. Freundlich isotherm is capable of demonstrating the effect of virus concentration on adsorption behavior. Langmuir isotherms can be used to compare relative adsorption among treatments, while the present study suggests that maximum adsorption can not be calculated when using the Langmuir isotherms.