玉米秸秆发酵浸出液模拟废水发酵产氢的放大实验研究

在20 L的半连续流发酵罐中，以牛粪堆肥为产氢菌源，按照玉米秸秆发酵浸出液的主要成分配制模拟废水，考察和分析了几个关键环境因素对发酵产氢的影响.结果表明，HRT、C/N、Fe²⁺浓度和模拟废水浓度对发酵产氢均有不同程度的影响.在本实验条件下，秸秆模拟废水的氢产量、氢浓度和产氢速率分别为11.80 mol/kg、56%和8.81 L/(L·d)，底物转化率大于90%，废水中COD去除率为39.40%.在整个发酵产氢过程，液相主要发酵副产物为丁酸、乙酸和丙酸以及少量的乙醇和丁醇.

Enlargement Test Studies of Bio-hydrogen Production Using Artificial Wastewater of Corn Stalk Fermentation Lixivium by Mixed Culture

In transient conditions close to the industrialized application situation, the removal of toluene was investigated with a lab-scale bio-trickling filter inoculated with pure bacterial culture (Pseudomonas putida). The start-up process and the ability of resisting different toluene loading in the steady state on the performance of the bio-trickling filter were studied. The microstructure of biofilm in the filter was also observed. With inlet concentration range from 544 to 1044 mg x m(-3) at the temperature ranging from 17 to 26 degrees C, the removal efficiency of toluene was almost 100% at the residence time of 54 s and 43.2 s. The maximum volumetric removal loading of 105.35 g x (m3 x h)(-1) was achieved. The results indicate that it was feasible to remove toluene by Pseudomonas putida which had not be acclimated by toluene. In the steady state, the bio-trickling filter had a high flexibility for the load change and the removal efficiency of the reactor was not influenced by the variance of residence time and inlet concentration. The rapid increase of biofilm can be controlled by adjusting the interval of nutrition liquid accession. There were some changes in bacterial community, and lots of micro-pore existed in the biofilm. It was proved that the absorption of the biofilm was an important precondition for the biodegradation of toluene.