一株香蒲根际土壤脱氮菌株的脱氮特性及鉴定

从南四湖人工湿地植物香蒲根际土壤中分离得到一株反硝化XP1菌株,对其生态影响因子、反硝化能力、生长曲线进行了研究,并在实验室条件下利用菌株XP1反植于湿地植物香蒲根际土壤,考察对香蒲的强化脱氮作用.结果表明,XP1菌株反硝化作用的最佳碳源为柠檬酸钠;在pH值为7.0~9.0范围内有良好的脱氮效果,最佳pH值为8.0;最佳碳源与氮源比为7;最佳初始氮浓度为30mg/L.XP1菌株8h后进入指数增长期,最终总氮去除率可达90%以上.用该菌株强化香蒲脱氮,对于强化湿地脱氮工艺中亚硝酸盐的去除起主要作用,在相同条件下总氮去除率由46%提高到90%以上,强化脱氮作用明显,有良好的实际应用前景.通过形态观察,XP1菌株为短杆状革兰氏阴性菌,大小约(0.25~0.3)′(0.6~0.8)mm;经16S rRNA基因测序、系统发育树分析,XP1菌株为类芽孢杆菌(Paenibacillus sp.).

Denitrification characteristics and identification of a denitrifying bacteria inhabiting in typha rhizosphere soils.

One strain of denitrifying bacteria named XP1 was isolated from the rhizosphere soils of Typha in constructed wetland of Nansi Lake.Some physiological properties of XP1 were investigated,such as denitrification capacibility,ecological affecting factor and growth curve.A certain of purifying strain of XP1 was inoculated to the rhizosphere soil of Typha to determine its enhancement effects on denitrification.That citrate sodium was the most suitable carbon source for XP1 to carry out denitrification,and the s...