| 平菇漆酶的性质和应用研究 |
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| 引用本文： | 杨清香,王涛,王栋,李学梅.平菇漆酶的性质和应用研究[J].环境科学与技术,2008,31(9). |
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| 作者姓名： | 杨清香 王涛 王栋 李学梅 |
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| 作者单位： | 河南师范大学生命科学学院,河南,新乡,453007 |
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| 基金项目： | 教育部高校新世纪优秀人才支持计划，国家自然科学基金 |
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| 摘 要： | 通过采用硫酸铵分级沉淀对平菇菌种831和予6两菌种所产生的漆酶进行了初步分离,并对酶的性质进行了初步研究,研究表明它们的最适反应温度都在60℃,最适反应pH都在3.0,对831漆酶进行除酚和固定化脱色的研究表明,在最佳除酚条件下50℃,pH5.0,反应3h,对25mg/L单宁酸的去除率可达24.4%,在所研究的不同固定化材料中,以尼龙网为材料的固定化效果最好,固定化酶对50mg/L的活性艳蓝脱色率可达游离酶脱色率的76%,重复应用4次脱色率没有降低趋势,之后逐渐下降。
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| 关 键 词： | 平菇 漆酶 除酚 脱色 |
Characteristics and Application of Laccase from Pleurotus ostreatus |
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| YANG Qing-xiang,WANG Tao,WANG Dong,LI Xue-mei.Characteristics and Application of Laccase from Pleurotus ostreatus[J].Environmental Science and Technology,2008,31(9). |
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| Authors: | YANG Qing-xiang WANG Tao WANG Dong LI Xue-mei |
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| Abstract: | Using (NH4)2SO4 fractionation method, crude enzyme of laccase was obtained from Pleuotus ostreatus 831 and Yu-6. Characteristics of enzyme from the two strains were studied. Results indicated that their optimum temperature and pH were 60℃ and 3.0 respectively. Crude enzyme from strain 831 could decompose 25mg/L polyphenol 24.4% under the optimum condition of 50℃ and pH 5.0 within 3h. Among various enzyme immobilizing materials, nylon net was the best and the immobilized enzyme could effectively decolorize 50mg/L KNR with decolorization rate of 76% within 5h. Immobilized enzyme could keep constant decolorization activities at least in 4 cycles of test. |
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| Keywords: | Pleuotus ostreatus laccase depolyphenol decoloration |
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