| Abstract: | Our previous studies found that the glutathione(GSH) content in experimental animals reduced significantly with a dose-effect manner after the exposure of formaldehyde(FA)at the environmental levels. Moreover, the reduction of glutathione has no apparent protective effect for the genetic toxicity indicator DNA-protein crosslink (DPC) caused by formaldehyde. For an in-depth understanding on the combined effects of GSH and FA we undertook this study. Hela cells were used for experimental materials in vitro, the experimental group was divided into four groups: the control group, 250μM GSH Group,250μM formaldehyde group, 250μM formaldehyde and 250μM GSH combined group; Kun Ming mice were used for experimental materials in vivo, intraperitoneal injection was used for the exposure, which last for two weeks, the four experimental groups included control group, 1mM GSH group, 1mM formaldehyde group, and 1mM formaldehyde and 1mMGSH combined group. The in vitro and in vivo experimental results were similar, there were no significant differences between the control groups and GSH groups (p>0.05; p>0.05 ); the DPC induced by formaldehyde groups were significantly higher than that of control groups (p<0.01; p<0.05); the DPC induced by the combined groups were not only significantly higher than that of control groups (p<0.01; p<0.01) but also significantly higher than that of formaldehyde exposure group (p<0.05; p<0.01). The results suggest that GSH would not lead to the formation of DPC alone, but glutathione has a promotive effect on the DPC induced by formaldehyde. Meanwhile, the mechanism of the promotive effect was discussed in this paper; the authors suggest that the promotive effect of glutathione on formaldehyde molecules may be similar to the mechanism of glutathione on nitric molecules. |
