Biodegradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by a Tropical Marine Bacterium, Pseudoalteromonas sp. NRRL B-30083 |
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Authors: | Timothy D Leathers Nadathur S Govind Richard V Greene |
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Institution: | (1) U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Agricultural Research Service, Peoria, IL;(2) Department of Marine Sciences, University of Puerto Rico, Mayaguez, PR;(3) U.S. Department of Agriculture, Office of International Research Programs, Agricultural Research Service, Beltsville, MD, 20705 |
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Abstract: | Pseudoalteromonas sp. NRRL B-30083 was isolated as the predominant PHBV-degrading organism from a tropical marine environment. In complex medium, the isolate grew well at temperatures between 23°C and 33°C, with an optimal doubling time of about 30 min. NaCl was required at concentrations between 0.2 N and 0.8 N. Optimal pH levels for growth were between pH 6.5 and pH 8.5. Liquid cultures grew modestly on PHBV as a sole carbon source under optimal conditions, although PHBV depolymerase activity was not detected. |
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Keywords: | Biodegradation marine polyhydroxyalkanoates poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Pseudoalteromonas |
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