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Biodegradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by a Tropical Marine Bacterium, Pseudoalteromonas sp. NRRL B-30083
Authors:Timothy D Leathers  Nadathur S Govind  Richard V Greene
Institution:(1) U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Agricultural Research Service, Peoria, IL;(2) Department of Marine Sciences, University of Puerto Rico, Mayaguez, PR;(3) U.S. Department of Agriculture, Office of International Research Programs, Agricultural Research Service, Beltsville, MD, 20705
Abstract:Pseudoalteromonas sp. NRRL B-30083 was isolated as the predominant PHBV-degrading organism from a tropical marine environment. In complex medium, the isolate grew well at temperatures between 23°C and 33°C, with an optimal doubling time of about 30 min. NaCl was required at concentrations between 0.2 N and 0.8 N. Optimal pH levels for growth were between pH 6.5 and pH 8.5. Liquid cultures grew modestly on PHBV as a sole carbon source under optimal conditions, although PHBV depolymerase activity was not detected.
Keywords:Biodegradation  marine  polyhydroxyalkanoates  poly(3-hydroxybutyrate-co-3-hydroxyvalerate)  Pseudoalteromonas
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