| 酶联免疫吸附分析法测定环境水体中的萘 |
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| 引用本文： | 杨丽波,庄惠生,周纯,叶其炎. 酶联免疫吸附分析法测定环境水体中的萘[J]. 化工环保, 2009, 29(6) |
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| 作者姓名： | 杨丽波 庄惠生 周纯 叶其炎 |
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| 作者单位： | 东华大学,环境科学与工程学院,上海,201620;东华大学,环境科学与工程学院,上海,201620;上海交通大学,环境科学与工程学院,上海,200240 |
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| 基金项目： | 国家自然科学基金，上海市重点学科建设项目 |
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| 摘 要： | 使用自制的包被原和抗萘多克隆兔抗体,建立了测定环境水体中痕量萘的间接竞争酶联免疫吸附法,并优化了实验条件.工作曲线线性方程为I=8.394logρ+50.21(I为抑制率,ρ为萘的质量浓度),线性范围10~(-3)～10 μg/L内的相关系数为0.985 6,方法灵敏度为0.944 μg/L,检出限为0.250 ng/L,回收率为86.00%～106.00%,批间误差为5.00%～15.00%,批内误差为2.00%～8.00%,与四种结构类似物的交叉率均小于14.0%.
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| 关 键 词： | 酶联免疫吸附法 萘 分析方法 |
Determination of Naphthalene in Environmental Water Bodies by Enzyme-linked Immunosorbent Assay Method |
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| Yang Libo,Zhuang Huisheng,Zhou Chun,Ye Qiyan. Determination of Naphthalene in Environmental Water Bodies by Enzyme-linked Immunosorbent Assay Method[J]. Environmental Protection of Chemical Industry, 2009, 29(6) |
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| Authors: | Yang Libo Zhuang Huisheng Zhou Chun Ye Qiyan |
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| Abstract: | A new enzyme-linked immunosorbent assay (ELISA) method for determination of trace naphthalene (NA) in environmental water bodies was developed using self-made coating antigen and rabbit polyclonal anti-naphthalene antibodies, and its operation conditions were optimized. The regression equation of the standard ELISA inhibition curve is I=8.394×logρ+50.21 (I for inhibition rate and ρ for NA mass concentration). The linearity range is 10~(-3)-10 μg/L. The correlation coefficient is 0.985 6. The sensitivity is 0.944 μg/L. The detection limit is 0.250 ng/L. The recovery is 86.00%-106.00%. The relative standard deviations of within-run and between-run are 2.00%-8.00%, 5.00%-15.00%, respectively. The cross reaction rates with four structurally analogues are all below 14.0%. |
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| Keywords: | enzyme-linked immunosorbent assay method naphthalene analysis method |
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