海洋细菌产淀粉酶条件的研究

采用3,5-二硝基水杨酸显色法测定淀粉酶的酶活,研究碳源、氮源、各种理化因素等培养条件对细菌产淀粉酶的影响.结果表明,最佳碳源为麸皮,最佳氮源为蛋白胨,最适初始PH值为7.90-8.20,培养温度31℃,在装液量为100mL三角瓶中装液40mL.通过对发酵条件的优化,酶活比未优化前提高了2.71倍.

Study on Fermentation Conditions of an Amylase-producing Marine Bacterium

The enzyme activity was finally determined with the chtomogenic method of 3,5-dinitrosalicylic acid,the optimal fermenting conditions of amylase produced by the strain were studied,including the carbon source,the nitrogen source,the initial pH,liquid volume,fermentation temperature.Bran and peptone were the best carbon source and nitrogen source,initial pH 7.90-8.20.Fermentation temperature were 31℃,cultivated in 40mL medium in 100mL shake flask.Clearing the effect of different fermentation conditions on an amylase-producing marine bacterium,under the optimum conditions the amylase activity was 2.71 times higher than that under the original conditions.