| 用蛋白磷酸酶抑制法测定水体中的微囊藻毒素类物质 |
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| 引用本文： | 徐立红,陈加平,许建明,张甬元,黄兆辉,林群声. 用蛋白磷酸酶抑制法测定水体中的微囊藻毒素类物质[J]. 环境科学学报, 1999, 19(5): 536-539 |
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| 作者姓名： | 徐立红 陈加平 许建明 张甬元 黄兆辉 林群声 |
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| 作者单位： | 1. 中国科学院水生生物研究所,武汉,430072
2. 香港城市大学生物及化学系,香港 |
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| 基金项目： | 国家自然科学基金，香港研究资助局资助，淡水生态与生物技术国家重点实验室资助 |
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| 摘 要： | 建立了一种灵敏的测定水体中微囊藻毒素类物质的方法。从鱼肝脏中分离的蛋白磷酸酶２Ａ被微囊藻毒素抑制，并且抑制与毒素剂量相关，抑制模式表现为典型的Ｓ型曲线。根据末知样品存在时蛋白磷酸酶的相对活力，可由本研究得出的标准曲线及公式，计算样品中可能存在的微囊藻毒素类物质。研究表明，在一年的观测周期内，即使没有可见的水华发生，东湖及鱼池中仍有可检测出微囊藻毒素类物质。
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| 关 键 词： | 水样 微囊藻毒素 蛋白磷酸酶抑制法 |
| 收稿时间： | 1997-12-22 |
| 修稿时间： | 1998-06-18 |
Use of protein phosphatase inhibition assay for measuring microcystin class compounds in aquatic system |
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| XU Lihong,CHEN Jiaping,XU Jianming,ZHANG Yongyuan,Bryan S F,Wang and Paul K S Lam. Use of protein phosphatase inhibition assay for measuring microcystin class compounds in aquatic system[J]. Acta Scientiae Circumstantiae, 1999, 19(5): 536-539 |
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| Authors: | XU Lihong CHEN Jiaping XU Jianming ZHANG Yongyuan Bryan S F Wang Paul K S Lam |
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| Affiliation: | Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072,Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072,Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072,Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072,Department of Biology and Chemistry, City University of Hong Kong, Hong Kong and Department of Biology and Chemistry, City University of Hong Kong, Hong Kong |
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| Abstract: | A sensitive method for detecting microcystin class compunds is established. Activities of protein phosphatase 2A isolated from fish liver were inhibited dose|dependenly by microcystins. The inhibition pattern showed a typical sigmoid curve. From the relative activities of protein phosphatase in the presence of unknown sample, the concentration of inhibitor in the sample could be calculated by using standard curve. Microcystin class compund could be detected from Donghu Lake and fish pond even no visible water bloom could be seen in the period of one year. |
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| Keywords: | microcystin water sample protein phosphatases inhibition assay. |
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