荧光法监测硝普钠释放NO过程及其对微藻生长的影响（Fluorescence Dynamic Monitoring of Nitric Oxide Release Process Following Administration of Sodium Nitroprusside and Its Effect on the Growth of Microalga）

以硝普钠(Sodium nitroprusside,SNP)为一氧化氮(Nitric oxide,NO)供体研究了NO对海洋微藻生长的影响.对不同浓度SNP在海水介质中释放NO的过程进行了监测;对所培养的亚心形扁藻(Platymonas subcordiformis)和中肋骨条藻(Skeletonema costatum)进行藻密度测定,观测NO对微藻生长的影响.结果表明:5、10和100μmol·L-1的SNP释放NO浓度大约分别为6×10-9、9×10-9和2×10-7mol·L-1左右,而释放时间分别为4、5.5和7.5h.研究表明,NO对不同微藻有明显不同的作用规律:NO持续作用下,对亚心形扁藻的最佳作用浓度在10-8mol·L-1数量级;对赤潮藻中肋骨条藻的最佳作用浓度在10-9mol·L-1数量级;赤潮藻对NO的响应比非赤潮藻更灵敏,NO可能是海洋生态系中微藻生长重要的调节因子.

Fluorescence Dynamic Monitoring of Nitric Oxide Release Process Following Administration of Sodium Nitroprusside and Its Effect on the Growth of Microalga

The effect of nitric oxide(NO),provided by sodium nitroprusside(SNP),on marine microalga growth was studied.Researches were conducted on the process of the release of nitric oxide in different concentrations of SNP in the seawater media.At the same time,microalga density of cultivated Platymonas subcordiformis and Skeletonema costatum were measured to estimate the influence of different concentrations of NO on the growth of microalgae.The results were as follows: The NO concentrations released by 5,10 and 100μmol·L-1 SNP were about the order of magnitude of 6×10-9,9×10-9 and 2×10-7mol·L-1,respectively.The time of NO release was about 4,5.5 and 7.5h,respectively.The results showed that NO had different effects on different microalga.Under the condition of continuous affection by NO,the best concentration of positive effect of NO on Platymonas subcordiformis was about 10-8mol·L-1 magnitude of order,while that for red tide algae Skeletonema costatum was about 10-9mol·L-1 magnitude of order.Red tide algae is more sensitive to NO than food alga.Maybe NO is the important regulator of the microalga growth in marine ecosystem.