Control strategies for biohydrogen production by immobilized co-culture of Clostridium butyricum and Rhodopseudomonas palustris |
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Authors: | Po-Min Kao Chi-Wei Tao Tsui-Kang Hsu Chia-Ming Chang |
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Institution: | 1. Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan;2. Department of Ophthalmology, Cheng Hsin General Hospital, Taipei, Taiwan;3. Department of Life Science, Institute of Molecular Biology, National Chung Cheng University, Chiayi, Taiwan |
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Abstract: | In this study, we evaluated biohydrogen production of Clostridium butyricum and Rhodopseudomonas palustris by immobilized co-culture. Effects of free cells and immobilized cells, immobilized biomass ratio, sucrose concentration, and initial pH on hydrogen production were investigated. The immobilized co-culture can achieve high cumulative hydrogen volume yield (604 mL) as compared to free co-culture cumulative hydrogen volume (513 mL) while the initial sucrose concentration was 17.8 g/L. The optimum C. butyricum/R. palustris ratio was 1:10, yielding the highest cumulative hydrogen (728 mL). High sucrose concentration (above 35.6 g/L) would inhibit hydrogen production. The optimal initial pH value for hydrogen production of immobilized co-culture was 7.0 (cumulative hydrogen volume 830 mL). |
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Keywords: | Anaerobic processes biohydrogen co-culture immobilized cells submerged culture sucrose |
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