Degradation of Polylactide by Commercial Proteases |
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| Authors: | Y Oda A Yonetsu T Urakami K Tonomura |
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| Institution: | (1) Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan;(2) Department of Food Science and Nutrition, Kinki University, Nara, Naka, 631-8505, Japan;(3) Mitsubishi Gas Chemical Company, Chiyoda-Ku, Tokyo, 100-8324, Japan;(4) Department of Applied Biological Science, Fukuyama University, Fukuyama, Hiroshima, 729-0292, Japan |
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| Abstract: | Fifty-six commercially available proteases were tested for polylactide-degrading activity. Little or no activity was found in acid and neutral proteases, while some alkaline proteases formed appreciable amounts of lactic acid from polylactide. These polylactide-degrading proteases were derived from Bacillus species and had catalytic activity even under neutral, as well as alkaline, conditions. Savinase (Novo Nordisk) degraded polylactide the fastest among the enzymes tested and its specific activity corresponded to about one-half of proteinase K. Polylactide-degrading activity was not always present in the enzymes that affected keratin, while polylactide-degrading proteases commonly hydrolyzed keratin. A significant correlation was observed between degrading activities of polylactide and keratin in alkaline proteases. |
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| Keywords: | Bacillus sp lactic acid polylactide protease proteinase K savinase |
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