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| 异养硝化/好氧反硝化菌的分离鉴定及其在不同培养条件下产N_2O研究 | |
| 引用本文： | 蒋静艳,胡正华,黄耀.异养硝化/好氧反硝化菌的分离鉴定及其在不同培养条件下产N_2O研究[J].环境科学,2009,30(7). |
| 作者姓名： | 蒋静艳 胡正华 黄耀 |
| 作者单位： | 1. 南京农业大学资源与环境科学学院,南京,210095 2. 南京信息工程大学环境科学与工程学院,南京,210044 3. 南京农业大学资源与环境科学学院,南京,210095;中国科学院大气物理研究所,北京,100029 |
| 基金项目： | 国家自然科学基金项目(40605029) |
| 摘 要： | 以乙酰胺为唯一碳氮源分离纯化出2株既能异养硝化又能好氧反硝化的菌株XM1和HX2,革兰氏阴性,分别为链状杆菌和球状菌.在富集培养基中,菌株HX2产N2O量为XM1的76倍;两菌株均能分别以葡萄糖、甘露醇、酒石酸钠为唯一碳源进行生长;也可分别以硝酸钠和硫酸铵为唯一氮源进行硝化和反硝化作用,但菌株XM1生长速率快于HX2,且有较多的NO2-积累.部分长度的16S rDNA序列分析表明,菌株XM1与HX2的序列与Pseudomonas sp.具有99%的相似性,定为假单胞菌属(Pseudomonas sp.).两菌株27d反接种培养实验结果表明,在添加外源N的条件下,HX2菌株更适合在30%的土壤水分(WFPS)条件下生长,N2O产生量为(36.01±2.48)ng/g,为60%WFPS条件下N2O产生量的1.9倍;而菌株HX1则适合在60%的水分条件下生长,在该条件下几乎不产生N2O. |
| 关 键 词： | 异养硝化/好氧反硝化 分离 N2O产生 |
Isolation of Heterotrophic Nitrifiers/Aerobic Denitrifiers and Their Roles in N_2O Production for Different Incubations |
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| JIANG Jing-yan,HU Zheng-hua,HUANG Yao.Isolation of Heterotrophic Nitrifiers/Aerobic Denitrifiers and Their Roles in N_2O Production for Different Incubations[J].Chinese Journal of Environmental Science,2009,30(7). | |
| Authors: | JIANG Jing-yan HU Zheng-hua HUANG Yao |
| Institution: | 1.College of Resources and Environmental Sciences;Nanjing Agricultural University;Nanjing 210095;China;2.Institute of Atmospheric Physics;Chinese Academy of Sciences;Beijing 100029;3.College of Environmental Science and Engineering;Nanjing University of Information Sciences & Technology;Nanjing 210044;China |
| Abstract: | Soil microorganisms are important sources of N2O for the atmosphere. Peak emissions of N2O are often observed after wetting of soil. The simultaneous heterotrophic nitrifying and aerobic denitrifying bacteria with respect to N2O emission were studied to obtain more information about the microbiological aspects of peak emissions. Using acetamide as the C and N source,two strains of nitrifying and denitrifying bacteria were isolated,coded as XM1 and HX2,respectively. XM1strain was Gram-negative chain-like bac... |
| Keywords: | heterotrophic nitrifier/aerobic denitrifier isolate N2O production |
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