石油烃降解混合菌的筛选及其降解条件研究

对采集克拉玛依地区的部分石油污染样品进行了富集分离,得到了5组石油烃高效降解混合菌,其中混合菌KL9-1对温度的耐受范围最宽,并且石油烃的降解效率最高。该混合菌在45℃的条件下,通过7 d的降解,稀油的降解率达到43.27%,稠油的降解率达到20.09%。利用单因素试验考察环境因素对混合菌KL9-1降解石油烃的影响,结果表明混合菌KL9-1的接种量、石油烃仞始浓度、初始pH、摇床转速、表面活性剂的添加都会影响石油烃的降解效果,在35℃的条件下,当接种量6.0%、石油烃初始浓度1.5%、仞始pH 7.5、摇床转速120 r/min及添加200 mg/kg Tween80表面活性剂时,稀油和稠油的降解率都达到最高,其中稀油的降解率可以达到62.49%,稠油的降解率达到40.36%。

Screening of a Hydrocarbon-degrading Bacterial Group and Study on Its Degrading Conditions

5 highly efficient hydrocarbon-degrading mixed bacteria were,obtained from petroleum-contaminated samples of Karamay,Xinjiang by the traditional method of enrichment and isolation,and it was found that KL9-1 group has a wide temperature tolerance range and higher hydrocarbon degrading ability.The degradation rate of thin oil and heavy oil was up to 43.27%and 20.09%respectively through 7d at 45℃.The environmental factors on the degradation of petroleum hydrocarbon effect of KL9-1 group were studied using single factor test.Experimental results indicated that the inoculums amount of KL9-1,initial concentration of petroleum hydrocarbons,initial pH value,shaking speed and adding amount of surfactant can affect the degradation efficiency of petroleum hydrocarbon.The degradation rate of thin oil and heavy oil was up to 62.49%and 40.36%respectively at 35℃under the conditions of inoculums amount 6.0%,initial concentration of petroleum hydrocarbons 1.5%,initial pH value 7.5,rotation speed 120r/min and adding 200 mg/kg Tween80.