生物强化水解酸化过程前后微生物群落结构变化

通过利用生物强化技术提高水解酸化处理模拟印染废水的处理效果,包括共基质强化和菌剂强化两个方面。菌剂和共基质强化结果表明,菌剂A和菌剂B的加入将脱色率分别提高了2.33%和7.20%,当不加碳源时,脱色效果比较差(低于6.00%),而蔗糖的加入能提高脱色率至45.29%。利用PCR-DGGE技术追踪生物强化前后微生物群落结构信息,结果表明,菌剂和共基质的加入都对水解酸化池的微生物群落结构有较大影响。菌剂A和B强化后,水解酸化池的微生物多样性指数H(Shannon Weaver)由2.38分别增长到2.56和2.69,而共基质调控使得H由2.94增加到3.16。菌剂强化后,优势菌厚壁菌(Firmicutes)得到强化。通过共基质强化,优势菌拟杆菌(Bacteroidetes)和厚壁菌(Firmicutes)得到强化,它们可能在染料降解中起到重要作用。

Changes of microbial community in hydrolysis acidification processs before and after bioaugmentation

Bioaugmentation technologies were used to improve the performance of hydrolysis acidification process treating simulated dyeing wastewater, including mixed flora and co-substrate bioaugmentation. The results indicate that with the augmentation by mixed flora A and B, the average decolorization rate of hydrolysis acidification process increases by 2.33% and 7.20%, respectively. Without co-substrate, the decolorization rate is less than 6.00%. While with sucrose as co-substrate, the decolorization rate increses to 45.29%. PCR-DGGE analysis suggests that the addition of mixed flora A and B and co-substrate have significant influence on the microbial community structure and microbial diversity in hydrolysis acidification reactor. After augmentation with mixed flora A and B, the Shannon Weaver index (H) increases from 2.38 to 2.56 and 2.69, respectively. Similarly, with co-substrate bioaugmentation, H increases from 2.94 to 3.16. The phylogenesis analysis reveals that with the augmentation by mixed flora A and B, dominant bacterial community Firmicutes are stimulated. Through the augmentation with co-substrate (sucrose), dominant bacterial community Bacteroidetes and Firmicutes are enhanced. They may play important roles in dyes degradation.