16SrDNA克隆文库分析含硫污水生物反应器中生物群落

利用生物脱硫技术处理炼油厂含硫污水,硫化物选择性地氧化成单质硫。在进水硫化物605.3 mg·L-1的条件下,硫化物去除率90.7%,单质硫生成率达到80.8%。提取反应器中的DNA样本,构建16S rDNA克隆文库对反应器中的生物群落进行分析。对51个阳性克隆子(长度约为1 400 bp)进行测序,测序结果在NCBI数据库利用Blast比对并构建系统发育树。测序结果表明,生物脱硫反应器细菌种群可分为10个类群,数量最多的是γ-变形菌(γ-Proteobacteria)占76.6%,其余类群共占23.4%。γ-Proteobacteria类群中数量最多的是具有脱硫功能的Halothiobacillus sp.(66.7%)、Thiovirga sp.(25.6%)以及Pseudomonas sp.(7.7%)。系统发育树分析结果表明,生物脱硫系统中形成了以化能自养型脱硫菌为主的微生物群落结构,异养菌数量较少。

Analysis of biological communities in bioreactor of treating sulfide-containing wastewater by 16S rDNA cloning method

Sulfide in oil refinery wastewater was selectively oxidized to sulfur in a biological desulfurization reactor.When sulfide concentration in the influent was 605.3 mg·L-1,90.7% sulfide removal was achieved and 80.8% of sulfide was converted into sulfur.DNA was extracted from the reactor to construct a 16S rDNA gene library for the analysis of biological communities.Fifty-one clones were randomly selected and their partial 16S rDNA(1 400 bp) was sequenced.Sequencing results were analyzed using the BLAST algorithm at NCBI and a phylogenetic tree was constructed,identifying 10 bacterial groups.The dominant bacterial community in the reactor is γ-Proteobacteria,which account for 76.6% of the sequences.The majority of γ-Proteobacteria belong to desulfurizing bacteria,such as Halothiobacillus sp.(66.7%),Thiovirga sp.(25.6%) and Pseudomonas sp.(7.7%).The phylogenetic analysis demonstrates that the desulfurization system has evolved into a unique microbial community structure consisting mainly of chemoautotrophic desulfurizing bacteria,rather than heterotrophic bacteria.