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Isolation and characterization of facultative mixotrophic ammonia-oxidizing bacteria from constructed wetlands
Authors:Kouki Soulwène  Saidi Neila  M'hiri Fadhel  Nasr Houda  Cherif Hanène  Ouzari Hadda  Hassen Abdennaceur
Affiliation:1. Laboratory of Treatment and Water Recycling, Centre of Research and Water Technologies,Technopark of Borj-Cedria, BP 273, 8020, Soliman, Tunisia
2. Unit of Transfer and Innovation Technologies, Tunis International Centre for Environmental Technologies,Boulevard of the Leader Yasser Arafat, La Charguia, 1080, Tunis, Tunisia
3. Laboratory of Microbiology and Active Biomolecules, Department of Biology, Faculty of Sciences of Tunis, 2092, Tunis, Tunisia
Abstract:Autotrophic ammonia-oxidizing bacteria (AOB) have been widely studied in constructed wetlands systems,while mixotrophic AOB have been less thoroughly examined.Heterotrophic bacteria were isolated from wastewater and rhizospheres of macrophytes of constructed wetlands,and then cultivated in a mixotrophic medium containing ammonium and acetic acid.A molecular characterization was accomplished using ITS-PCR amplification,and phylogenetic analysis based on 16S rRNA gene sequences.Results showed the presence of ...
Keywords:ammonia-oxidizing bacteria  mixotroph  constructed wetlands  ammonia oxidation  macrophytes' rhizosphere
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